Abstract
Topiramate (Topamax®) is an antiepileptic medication used as adjunctive and monotherapy in patients with epilepsy and for migraine prophylaxis. A GC-MS assay was developed that was capable of detecting topiramate plasma concentrations following a single rectal or oral dose administration. Topiramate plasma samples were prepared by solid-phase extraction and were quantified by GC-MS analysis. The topiramate standard curves were split from 0.1 to 4 μg/mL and from 4 to 40 μg/mL in order to give a more accurate determination of the topiramate concentration. The accuracy of the standards ranged from 94.6 to 107.3% and the precision (%CV) ranged from 1.0 to 5.3% for both curves at all concentrations. The %CV for quality controls was <7.6%. The assay is both accurate and precise and will be used to complete future pharmacokinetic studies.
Original language | English (US) |
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Pages (from-to) | 1071-1076 |
Number of pages | 6 |
Journal | Biomedical Chromatography |
Volume | 26 |
Issue number | 9 |
DOIs | |
State | Published - Sep 2012 |
Keywords
- Cyheptamide
- Gas chromatography
- Humans
- Mass spectrometry
- Topiramate