A simple method for concentration of enteroviruses and rotaviruses from cell culture harvests using membrane filters

S. R. Farrah; S. M. Goyal; C. P. Gerba; R. H. Conklin; C. Wallis; J. L. Melnick; H. L. DuPont

doi:10.1159/000148921

A simple method for concentration of enteroviruses and rotaviruses from cell culture harvests using membrane filters

S. R. Farrah, S. M. Goyal, C. P. Gerba, R. H. Conklin, C. Wallis, J. L. Melnick, H. L. DuPont

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Organic compounds in cell culture harvests known as membrane coating components (MCC) prevent virus adsorption to membrane filters. Blending cell culture harvests with fluorocarbon removed the MCC and permitted adsorption of virus in acidified harvests to epoxy fiberglass filters. Subsequent elution with pH buffer resulted in recovery of greater than 90% of the virus with concentrations of up to 100 fold.

Original language	English (US)
Pages (from-to)	56-59
Number of pages	4
Journal	Unknown Journal
Volume	9
Issue number	1
DOIs	https://doi.org/10.1159/000148921
State	Published - 1978
Externally published	Yes

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title = "A simple method for concentration of enteroviruses and rotaviruses from cell culture harvests using membrane filters",

abstract = "Organic compounds in cell culture harvests known as membrane coating components (MCC) prevent virus adsorption to membrane filters. Blending cell culture harvests with fluorocarbon removed the MCC and permitted adsorption of virus in acidified harvests to epoxy fiberglass filters. Subsequent elution with pH buffer resulted in recovery of greater than 90% of the virus with concentrations of up to 100 fold.",

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AU - Farrah, S. R.

AU - Goyal, S. M.

AU - Gerba, C. P.

AU - Conklin, R. H.

AU - Wallis, C.

AU - Melnick, J. L.

AU - DuPont, H. L.

PY - 1978

Y1 - 1978

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AB - Organic compounds in cell culture harvests known as membrane coating components (MCC) prevent virus adsorption to membrane filters. Blending cell culture harvests with fluorocarbon removed the MCC and permitted adsorption of virus in acidified harvests to epoxy fiberglass filters. Subsequent elution with pH buffer resulted in recovery of greater than 90% of the virus with concentrations of up to 100 fold.

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A simple method for concentration of enteroviruses and rotaviruses from cell culture harvests using membrane filters

Abstract

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