A Stromal Niche Defined by Expression of the Transcription Factor WT1 Mediates Programming and Homeostasis of Cavity-Resident Macrophages

Matthew B. Buechler, Ki Wook Kim, Emily J. Onufer, Jesse W. Williams, Christine C. Little, Claudia X. Dominguez, Qingling Li, Wendy Sandoval, Jonathan E. Cooper, Charles A. Harris, Melissa R. Junttila, Gwendalyn J. Randolph, Shannon J. Turley

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Buechler et al. show that large cavity macrophages (LCMs), which rely on the retinoic-acid-responsive transcription factor GATA6, are found in all visceral cavity spaces. Wt1+ mesothelial and fibroblastic stromal cells maintain the LCM transcriptome in part via the generation of retinoic acid and are required for LCM homeostasis in vivo.

Original languageEnglish (US)
Pages (from-to)119-130.e5
JournalImmunity
Volume51
Issue number1
DOIs
StatePublished - Jul 16 2019
Externally publishedYes

Bibliographical note

Funding Information:
We acknowledge members of the Turley and Randolph labs for their useful discussions and assistance in the laboratory. We thank facility staff at Genentech and Washington University in St. Louis for vivarium maintenance and core facility assistance and N.W. Lounsbury and S. Lianoglou for management of sequencing files. We express gratitude to A.M. Berkley for her careful review of this manuscript. We also acknowledge technical support from BioRender for the graphical abstract. This work was supported by Genentech, NIH grants R37 AI049653 and DP1DK109668 to G.J.R., K99 HL138163 to J.W.W., and T32 DK077653 to E.J.O.

Publisher Copyright:
© 2019 Elsevier Inc.

Keywords

  • Fibroblasts
  • Macrophages
  • Mesothelial cells
  • Retinoic acid
  • WT1

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