A versatile multimode microscope to probe and manipulate nanoparticles and biomolecules

M. Kyoung, K. Karunwi, E. D. Sheets

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

We describe a flexible, multifaceted optical setup that allows quantitative measurement and manipulation of biomolecules and nanoparticles in biomimetic and cellular systems. We have implemented integrated biophotonics techniques (i.e. differential interference contrast, wide-field fluorescence, prism- and objective-based total internal reflection excitation, single particle tracking, fluorescence correlation spectroscopy and dynamic holographic optical trapping) on a single platform. The adaptability of this versatile, custom-designed system allows us to simultaneously monitor cell morphology, while measuring lateral diffusion of biomolecules or controlling their cellular location or interaction partners.

Original languageEnglish (US)
Pages (from-to)137-146
Number of pages10
JournalJournal of Microscopy
Volume225
Issue number2
DOIs
StatePublished - Feb 1 2007

Keywords

  • Fluorescence correlation spectroscopy
  • Fluorescence microscopy
  • Holographic optical trap
  • Single particle tracking
  • Total internal reflection fluorescence

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