Accuracy of the Zika IgM antibody capture enzyme-linked immunosorbent assay from the centers for disease control and prevention (CDC Zika MAC-ELISA) for diagnosis of zika virus infection

Moyra Machado Portilho, Laise de Moraes, Mariana Kikuti, Leile Camila Jacob Nascimento, Mitermayer Galvão Reis, Viviane Sampaio Boaventura, Ricardo Khouri, Guilherme Sousa Ribeiro

Research output: Contribution to journalArticlepeer-review

Abstract

Serological diagnosis of Zika virus (ZIKV) infection is challenging because of antigenic cross-reactivity with dengue virus (DENV). This study evaluated the accuracy of the Zika IgM antibody capture enzyme-linked immunosorbent assay (CDC Zika IgM MAC-ELISA) in differentiating between ZIKV and DENV infections. To determine sensitivity, we used acute- and convalescent-phase sera from 21 patients with RT-PCR-confirmed ZIKV infection. To determine specificity, we used acute- and convalescent-phase sera from 60 RT-PCR-confirmed dengue cases and sera from 23 blood donors. During the acute-phase of the illness, the assay presented a sensitivity of 12.5% (2/16) for samples collected 0–4 days post symptoms onset (DPSO), and of 75.0% (3/4) for samples collected 5–9 DPSO. During the convalescent-phase of the illness, the test sensitivity was 90.9% (10/11), 100% (2/2), and 0% (0/2) for samples obtained 12–102, 258–260, and 722–727 DPSO, respectively. Specificity for acute- and convalescent-phase samples from RT-PCR-confirmed dengue cases was 100% and 93.2%, respectively. Specificity for blood donor samples was 100%. The assay is an accurate method for Zika serological diagnosis and proved to be reliable for use during surveillance and outbreak investigations in settings where ZIKV and DENV cocirculate.

Original languageEnglish (US)
Article number835
JournalDiagnostics
Volume10
Issue number10
DOIs
StatePublished - Oct 18 2020
Externally publishedYes

Bibliographical note

Funding Information:
Funding: This study was supported by the Brazilian National Council for Scientific and Technological Development (grant 550160/2010-8 to MGR, grants 400830/2013-2 and 440891/2016-7 to GSR, grant 439967/2016-3 to RK, and scholarships to MGR and GSR); the Bahia Foundation for Research Support (grant PNX0010/2011 to MGR, grants PPP0055/2011, JCB0020/2013, PET0026/2013, APP0044/2016, and PET0022/2016 to GSR); and the Coordination for the Improvement of Higher Education Personnel, Brazilian Ministry of Education (grant 88887.130746/2016-00 to GSR and scholarships to MMP, LM, MK and LCJN).

Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

  • CDC Zika MAC-ELISA
  • Diagnostic accuracy
  • Enzyme-linked immunosorbent assay
  • Sensitivity and specificity
  • Zika virus

Fingerprint Dive into the research topics of 'Accuracy of the Zika IgM antibody capture enzyme-linked immunosorbent assay from the centers for disease control and prevention (CDC Zika MAC-ELISA) for diagnosis of zika virus infection'. Together they form a unique fingerprint.

Cite this