Activin A maintains pluripotency of human embryonic stem cells in the absence of feeder layers

Gillian M. Beattie, Ana D. Lopez, Nathan Bucay, Andrew Hinton, Meri T. Firpo, Charles C. King, Alberto Hayek

Research output: Contribution to journalArticlepeer-review

397 Scopus citations

Abstract

To date, all human embryonic stem cells (hESCs) available for research require unidentified soluble factors secreted from feeder layers to maintain the undifferentiated state and pluripotency. Activation of STAT3 by leukemia inhibitory factor is required to maintain "stemness" in mouse embryonic stem cells, but not in hESCs, suggesting the existence of alternate signaling pathways for self-renewal and pluripotency in human cells. Here we show that activin A is secreted by mouse embryonic feeder layers (mEFs) and that culture medium enriched with activin A is capable of maintaining hESCs in the undifferentiated state for >20 passages without the need for feeder layers, conditioned medium from mEFs, or STAT3 activation. hESCs retained both normal karyotype and markers of undifferentiated cells, including Oct-4, nanog, and TRA-1-60 and remained pluripotent, as shown by the in vivo formation of teratomas.

Original languageEnglish (US)
Pages (from-to)489-495
Number of pages7
JournalSTEM CELLS
Volume23
Issue number4
DOIs
StatePublished - Apr 2005

Keywords

  • Activin A
  • Defined medium
  • Human embryonic stem cells
  • Pluripotency

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