Abstract
Adhesion- and degranulation-promoting adapter protein (ADAP) is required in TCR-induced activation and proliferation of peripheral T cells. Loss of ADAP also impairs TCR-initiated inside-out activation of the integrin LFA-1 (CD11a/CD18, αLβ2). In this study, we demonstrate that ADAP-deficient CD4/CD8 doubl-positive(DP) cells have a diminished ability to proliferate, and that these DP thymocytes up-regulate CD69 poorly in vivo. Moreover, in both MHC class I- and class II-restricted TCR transgenic models, loss of ADAP interferes with both positive and negative selection. ADAP deficiency also impairs the ability of transgene-bearing DP thymocytes to form conjugates with Ag-loaded presenting cells. These findings suggest that ADAP is critical for thymocyte development and selection.
Original language | English (US) |
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Pages (from-to) | 6681-6689 |
Number of pages | 9 |
Journal | Journal of Immunology |
Volume | 176 |
Issue number | 11 |
DOIs | |
State | Published - Jun 1 2006 |