Proteome characterization using mass spectrometry is essential for the systematic investigation of biological systems and for the study of gene function. Recent advances in this multifaceted field have occurred in four general areas: protein and peptide separation methodologies; selective labeling chemistries for quantitative measurement of peptide and protein abundances; characterization of post-translational protein modifications; and instrumentation.
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We apologize to those authors whose work could not be included here due to the constraints of space. We would also like to thank Sam Donohoe and Michael Wright for their assistance with the manuscript. TJG was funded by an NIH Postdoctoral Genome Training Grant fellowship. This work was also supported by grants from the National (USA) Cancer Institute (1R33CA84698) and from the NIH (RO1 AI41109-01).