Aggregation and Concentration-Dependent Sorting of Exocrine-Regulated Secretory Proteins

S. U. Gorr, S. Y. Tseng

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Sorting between the regulated and the constitutive secretory pathway in exocrine cells is thought to involve aggregation of regulated secretory proteins. This study demonstrates that, unlike endocrine secretory proteins, exocrine secretory proteins, including amylase, do not undergo homotypic aggregation under the conditions found in the sorting organelles. Also, unlike other exocrine proteins, amylase does not aggregate with chondroitin sulfate. Since amylase exhibits heterotypic aggregation, the role of protein concentration in amylase sorting was tested in AR42J cells, Secretion was stimulated with substance P and cholecystokinin from both untreated and dexamethasone-treated cells, with more efficient stimulation from dexamethasone-treated cells. These results indicate that amylase sorting is enhanced when its expression is stimulated by dexamethasone treatment.

Original languageEnglish (US)
Pages (from-to)82-88
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume215
Issue number1
DOIs
StatePublished - Oct 4 1995

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