Albumin adsorption onto surfaces of urine collection and analysis containers

Mary K. Robinson; Samuel P. Caudill; David D. Koch; James Ritchie; Glen Hortin; John H. Eckfeldt; Sverre Sandberg; Desmond Williams; Gary Myers; W. Greg Miller

doi:10.1016/j.cca.2014.01.035

Albumin adsorption onto surfaces of urine collection and analysis containers

Mary K. Robinson, Samuel P. Caudill, David D. Koch, James Ritchie, Glen Hortin, John H. Eckfeldt, Sverre Sandberg, Desmond Williams, Gary Myers, W. Greg Miller

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Background: Adsorption of albumin onto urine collection and analysis containers may cause falsely low concentrations. Methods: We added ¹²⁵I-labeled human serum albumin to urine and to phosphate buffered solutions, incubated them with 22 plastic container materials and measured adsorption by liquid scintillation counting. Results: Adsorption of urine albumin (UA) at 5-6. mg/l was <. 0.9%; and at 90. mg/l was <. 0.4%. Adsorption was generally less at pH. 8 than pH. 5 but only 3 cases had p. <. 0.05. Adsorption from 11 unaltered urine samples with albumin 5-333. mg/l was <. 0.8%. Albumin adsorption for the material with greatest binding was extrapolated to the surface areas of 100. ml and 2. l collection containers, and to instrument sample cups and showed <. 1% change in concentration at 5. mg/l and <. 0.5% change at 20. mg/l or higher concentrations. Adsorption of albumin from phosphate buffered solutions (2-28%) was larger than that from urine. Conclusions: Albumin adsorption differed among urine samples and plastic materials, but the total influence of adsorption was <. 1% for all materials and urine samples tested. Adsorption of albumin from phosphate buffered solutions was larger than that from urine and could be a limitation for preparations used as calibrators.

Original language	English (US)
Pages (from-to)	40-45
Number of pages	6
Journal	Clinica Chimica Acta
Volume	431
DOIs	https://doi.org/10.1016/j.cca.2014.01.035
State	Published - Apr 20 2014

Bibliographical note

Funding Information:
We thank the NKDEP-IFCC Joint Working Group for Standardization of Urinary Albumin for developing the framework, the National Center for Chronic Disease Prevention and Health Promotion at the CDC for providing funding, and the Newborn Screening and Molecular Biology Branch at the CDC for use of their radiation laboratory. We thank Elizabeth Monsell for assisting with the experimental design, Theodosia Prater for technical assistance and Pamela Olive for assisting with operation of the Hitachi 912. Appendix A

Keywords

Albumin adsorption
Urine albumin
Urine containers

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title = "Albumin adsorption onto surfaces of urine collection and analysis containers",

abstract = "Background: Adsorption of albumin onto urine collection and analysis containers may cause falsely low concentrations. Methods: We added 125I-labeled human serum albumin to urine and to phosphate buffered solutions, incubated them with 22 plastic container materials and measured adsorption by liquid scintillation counting. Results: Adsorption of urine albumin (UA) at 5-6. mg/l was <. 0.9%; and at 90. mg/l was <. 0.4%. Adsorption was generally less at pH. 8 than pH. 5 but only 3 cases had p. <. 0.05. Adsorption from 11 unaltered urine samples with albumin 5-333. mg/l was <. 0.8%. Albumin adsorption for the material with greatest binding was extrapolated to the surface areas of 100. ml and 2. l collection containers, and to instrument sample cups and showed <. 1% change in concentration at 5. mg/l and <. 0.5% change at 20. mg/l or higher concentrations. Adsorption of albumin from phosphate buffered solutions (2-28%) was larger than that from urine. Conclusions: Albumin adsorption differed among urine samples and plastic materials, but the total influence of adsorption was <. 1% for all materials and urine samples tested. Adsorption of albumin from phosphate buffered solutions was larger than that from urine and could be a limitation for preparations used as calibrators.",

keywords = "Albumin adsorption, Urine albumin, Urine containers",

author = "Robinson, {Mary K.} and Caudill, {Samuel P.} and Koch, {David D.} and James Ritchie and Glen Hortin and Eckfeldt, {John H.} and Sverre Sandberg and Desmond Williams and Gary Myers and Miller, {W. Greg}",

note = "Funding Information: We thank the NKDEP-IFCC Joint Working Group for Standardization of Urinary Albumin for developing the framework, the National Center for Chronic Disease Prevention and Health Promotion at the CDC for providing funding, and the Newborn Screening and Molecular Biology Branch at the CDC for use of their radiation laboratory. We thank Elizabeth Monsell for assisting with the experimental design, Theodosia Prater for technical assistance and Pamela Olive for assisting with operation of the Hitachi 912. Appendix A ",

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doi = "10.1016/j.cca.2014.01.035",

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AU - Robinson, Mary K.

AU - Caudill, Samuel P.

AU - Koch, David D.

AU - Ritchie, James

AU - Hortin, Glen

AU - Eckfeldt, John H.

AU - Sandberg, Sverre

AU - Williams, Desmond

AU - Myers, Gary

AU - Miller, W. Greg

N1 - Funding Information: We thank the NKDEP-IFCC Joint Working Group for Standardization of Urinary Albumin for developing the framework, the National Center for Chronic Disease Prevention and Health Promotion at the CDC for providing funding, and the Newborn Screening and Molecular Biology Branch at the CDC for use of their radiation laboratory. We thank Elizabeth Monsell for assisting with the experimental design, Theodosia Prater for technical assistance and Pamela Olive for assisting with operation of the Hitachi 912. Appendix A

PY - 2014/4/20

Y1 - 2014/4/20

N2 - Background: Adsorption of albumin onto urine collection and analysis containers may cause falsely low concentrations. Methods: We added 125I-labeled human serum albumin to urine and to phosphate buffered solutions, incubated them with 22 plastic container materials and measured adsorption by liquid scintillation counting. Results: Adsorption of urine albumin (UA) at 5-6. mg/l was <. 0.9%; and at 90. mg/l was <. 0.4%. Adsorption was generally less at pH. 8 than pH. 5 but only 3 cases had p. <. 0.05. Adsorption from 11 unaltered urine samples with albumin 5-333. mg/l was <. 0.8%. Albumin adsorption for the material with greatest binding was extrapolated to the surface areas of 100. ml and 2. l collection containers, and to instrument sample cups and showed <. 1% change in concentration at 5. mg/l and <. 0.5% change at 20. mg/l or higher concentrations. Adsorption of albumin from phosphate buffered solutions (2-28%) was larger than that from urine. Conclusions: Albumin adsorption differed among urine samples and plastic materials, but the total influence of adsorption was <. 1% for all materials and urine samples tested. Adsorption of albumin from phosphate buffered solutions was larger than that from urine and could be a limitation for preparations used as calibrators.

AB - Background: Adsorption of albumin onto urine collection and analysis containers may cause falsely low concentrations. Methods: We added 125I-labeled human serum albumin to urine and to phosphate buffered solutions, incubated them with 22 plastic container materials and measured adsorption by liquid scintillation counting. Results: Adsorption of urine albumin (UA) at 5-6. mg/l was <. 0.9%; and at 90. mg/l was <. 0.4%. Adsorption was generally less at pH. 8 than pH. 5 but only 3 cases had p. <. 0.05. Adsorption from 11 unaltered urine samples with albumin 5-333. mg/l was <. 0.8%. Albumin adsorption for the material with greatest binding was extrapolated to the surface areas of 100. ml and 2. l collection containers, and to instrument sample cups and showed <. 1% change in concentration at 5. mg/l and <. 0.5% change at 20. mg/l or higher concentrations. Adsorption of albumin from phosphate buffered solutions (2-28%) was larger than that from urine. Conclusions: Albumin adsorption differed among urine samples and plastic materials, but the total influence of adsorption was <. 1% for all materials and urine samples tested. Adsorption of albumin from phosphate buffered solutions was larger than that from urine and could be a limitation for preparations used as calibrators.

KW - Albumin adsorption

KW - Urine albumin

KW - Urine containers

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SP - 40

EP - 45

JO - Clinica Chimica Acta

JF - Clinica Chimica Acta

ER -

Albumin adsorption onto surfaces of urine collection and analysis containers

Abstract

Bibliographical note

Keywords

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