Alternative Ac/Ds transposition induces major chromosomal rearrangements in maize

Jianbo Zhang, Chuanhe Yu, Vinay Pulletikurti, Jonathan Lamb, Tatiana Danilova, David F. Weber, James Birehler, Thomas Peterson

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

Barbara McClintock reported that the Ac/Ds transposable element system can generate major chromosomal rearrangements (MCRs), but the underlying mechanism has not been determined. Here, we identified a series of chromosome rearrangements derived from maize lines containing pairs of closely linked Ac transposable element termini. Molecular and cytogenetic analyses showed that the MCRs in these lines comprised 17 reciprocal translocations and two large inversions. The breakpoints of all 19 MCRs are delineated by Ac termini and characteristic 8-base-pair target site duplications, indicating that the MCRs were generated by precise transposition reactions involving the Ac termini of two closely linked elements. This alternative transposition mechanism may have contributed to chromosome evolution and may also occur during V(D)J recombination resulting in oncogenic translocations.

Original languageEnglish (US)
Pages (from-to)755-765
Number of pages11
JournalGenes and Development
Volume23
Issue number6
DOIs
StatePublished - Mar 15 2009
Externally publishedYes

Keywords

  • Chromosome rearrangements
  • Hat elements
  • Transposition
  • V(D)J recombination

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