Abstract
Despite the utility of high performance liquid chromatography (HPLC) simulators, we found that all the free and low-cost simulators were outdated or had extremely limited functionality, so we created one that addressed these shortcomings. We developed a sophisticated, open-source HPLC simulator that is available for free as well as a version for Android users called "HPLC Simulator." Here we discuss a few questions that the simulator can help answer: Why are peaks narrower in gradient elution? What is gradient delay and how does it affect a separation? How much does extracolumn tubing affect a separation?.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 200-207 |
| Number of pages | 8 |
| Journal | LC-GC North America |
| Volume | 33 |
| Issue number | 3 |
| State | Published - 2015 |
Bibliographical note
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