TY - JOUR
T1 - An RNA polymerase III subunit determines sites of retrotransposon integration
AU - Bridier-Nahmias, Antoine
AU - Tchalikian-Cosson, Aurélie
AU - Baller, Joshua A.
AU - Menouni, Rachid
AU - Fayol, Hélène
AU - Flores, Amando
AU - Saïb, Ali
AU - Werner, Michel
AU - Voytas, Daniel F.
AU - Lesage, Pascale
PY - 2015/5/1
Y1 - 2015/5/1
N2 - Mobile genetic elements are ubiquitous. Their integration site influences genome stability and gene expression. The Ty1 retrotransposon of the yeast Saccharomyces cerevisiae integrates upstream of RNA polymerase III (Pol III)-transcribed genes, yet the primary determinant of target specificity has remained elusive. Here we describe an interaction between Ty1 integrase and the AC40 subunit of Pol III and demonstrate that AC40 is the predominant determinant targeting Ty1 integration upstream of Pol III-transcribed genes. Lack of an integrase-AC40 interaction dramatically alters target site choice, leading to a redistribution of Ty1 insertions in the genome, mainly to chromosome ends. The mechanism of target specificity allows Ty1 to proliferate and yet minimizes genetic damage to its host.
AB - Mobile genetic elements are ubiquitous. Their integration site influences genome stability and gene expression. The Ty1 retrotransposon of the yeast Saccharomyces cerevisiae integrates upstream of RNA polymerase III (Pol III)-transcribed genes, yet the primary determinant of target specificity has remained elusive. Here we describe an interaction between Ty1 integrase and the AC40 subunit of Pol III and demonstrate that AC40 is the predominant determinant targeting Ty1 integration upstream of Pol III-transcribed genes. Lack of an integrase-AC40 interaction dramatically alters target site choice, leading to a redistribution of Ty1 insertions in the genome, mainly to chromosome ends. The mechanism of target specificity allows Ty1 to proliferate and yet minimizes genetic damage to its host.
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U2 - 10.1126/science.1259114
DO - 10.1126/science.1259114
M3 - Article
C2 - 25931562
AN - SCOPUS:84929501317
SN - 0036-8075
VL - 348
SP - 585
EP - 588
JO - Science
JF - Science
IS - 6234
ER -