Analysis of the effector activity of Ara h 2 and Ara h 6 by selective depletion from a crude peanut extract

Xueni Chen, Yonghua Zhuang, Qian Wang, Daphne Moutsoglou, Glenn Ruiz, S. E. Yen, Stephen C. Dreskin

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

It is important to know the contribution of specific allergens to a complex allergenic extract and to have a dependable method to assess the effector activity of an extract specifically depleted of that allergen. We have previously shown that removal of the major peanut allergen, Ara h 2, from a crude peanut extract (CPE) minimally altered the effector activity of the extract. Here we describe in detail the methodology used to generate specific rabbit anti-peptide antibodies to remove a related peanut allergen, Ara h 6, from CPE and describe an improvement in the RBL SX-38 cell assay used to assess the effector activity of treated extracts. Our results show that although Ara h 2 and Ara h 6 can be selectively removed from a CPE, removal of each alone from a CPE had no significant effect on the effector activity. However, removal of Ara h 2 and Ara h 6 together significantly reduced the effector activity of CPE.

Original languageEnglish (US)
Pages (from-to)65-70
Number of pages6
JournalJournal of Immunological Methods
Volume372
Issue number1-2
DOIs
StatePublished - Sep 30 2011

Keywords

  • Allergen
  • Ara h 2
  • Ara h 6
  • IgE
  • Peanut allergy
  • Peanuts

Fingerprint Dive into the research topics of 'Analysis of the effector activity of Ara h 2 and Ara h 6 by selective depletion from a crude peanut extract'. Together they form a unique fingerprint.

Cite this