@inbook{d9812e2cd61e4818981128c64d8f3804,
title = "Analyzing origin activation patterns by copy number change experiments",
abstract = "Advances in microarray technology have enabled the analysis of replication dynamics on a genome-wide scale, providing deeper insight to the factors that regulate DNA replication. Studies using high-density microarrays have led to the genome-wide identification of replication origins in the budding yeast, Saccharomyces cerevisiae, and enabled the analysis of the global temporal pattern of origin activation under various conditions. We have developed a replication origin array that contains the ~430 potential origins in the yeast genome. By detecting the copy number change that occurs as cells progress from G1 to S phase on these arrays, we have produced origin activation patterns in wild-type cells similar to those obtained from previous studies that used whole-genome arrays. We have also applied this method to study S phase checkpoint mutants, providing insight into the genome-wide regulation of replication origin activation by S phase checkpoint kinases in the presence of replication stress. The main procedures of this technique involve arresting yeast cells in G1 and S phase, isolating and labeling genomic DNA with fluorescent dyes, and cohybridizing the DNA samples to replication origin arrays to yield copy number change data.",
keywords = "Copy number, DNA microarrays, Origin activation, Replication origins, S phase, Yeast",
author = "Miruthubashini Raveendranathan and Bielinsky, {Anja Katrin}",
year = "2009",
doi = "10.1007/978-1-60327-815-7_15",
language = "English (US)",
isbn = "9781603278140",
series = "Methods in Molecular Biology",
pages = "279--294",
booktitle = "DNA Replication",
}