Antagonism influences assembly of a Bacillus guild in a local community and is depicted as a food-chain network

Rocío Anaís Pérez-Gutiérrez; Varinia López-Ramírez; África Islas; Luis David Alcaraz; Ismael Hernández-González; Beatriz Carely Luna Olivera; Moisés Santillán; Luis E. Eguiarte; Valeria Souza; Michael Travisano; Gabriela Olmedo-Alvarez

doi:10.1038/ismej.2012.119

Antagonism influences assembly of a Bacillus guild in a local community and is depicted as a food-chain network

Rocío Anaís Pérez-Gutiérrez, Varinia López-Ramírez, África Islas, Luis David Alcaraz, Ismael Hernández-González, Beatriz Carely Luna Olivera, Moisés Santillán, Luis E. Eguiarte, Valeria Souza, Michael Travisano, Gabriela Olmedo-Alvarez

Ecology, Evolution and Behavior

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70 Scopus citations

Abstract

Understanding the principles that govern community assemblages is a central goal of ecology. There is limited experimental evidence in natural settings showing that microbial assembly in communities are influenced by antagonistic interactions. We, therefore, analyzed antagonism among bacterial isolates from a taxonomically related bacterial guild obtained from five sites in sediments from a fresh water system. We hypothesized that if antagonistic interactions acted as a shaping force of the community assembly, then the frequency of resistance to antagonism among bacterial isolates originating from a given site would be higher than the resistance to conspecifics originating from a different assemblage. Antagonism assays were conducted between 78 thermoresistant isolates, of which 72 were Bacillus spp. Sensitive, resistant and antagonistic isolates co-occurred at each site, but the within-site frequency of resistance observed was higher than that observed when assessed across-sites. We found that antagonism results from bacteriocin-like substances aimed at the exclusion of conspecifics. More than 6000 interactions were scored and described by a directed network with hierarchical structure that exhibited properties that resembled a food chain, where the different Bacillus taxonomic groups occupied specific positions. For some tested interacting pairs, the unidirectional interaction could be explained by competition that inhibited growth or completely excluded one of the pair members. This is the first report on the prevalence and specificity of Bacillus interactions in a natural setting and provides evidence for the influence of bacterial antagonist interactions in the assemblage of a taxonomically related guild in local communities.

Original language	English (US)
Pages (from-to)	487-497
Number of pages	11
Journal	ISME Journal
Volume	7
Issue number	3
DOIs	https://doi.org/10.1038/ismej.2012.119
State	Published - Mar 2013

Bibliographical note

Funding Information:
The support of the Namibian authorities and of the University of Namibia in facilitating the construction and operation of H.E.S.S. is gratefully acknowledged, as is the support by the German Ministry for Education and Research (BMBF), the Max Planck Society, the French Ministry for Research, the CNRS-IN2P3 and the Astroparticle Interdisciplinary Programme of the CNRS, the U.K. Science and Technology Facilities Council (STFC), the IPNP of Charles University, the Polish Ministry of Science and Higher Education, the South African Department of Science and Technology and National Research Foundation, and the University of Namibia. We appreciate the excellent work of the technical support staff in Berlin, Durham, Hamburg, Heidelberg, Palaiseau, Paris, Saclay, and Namibia in the construction and operation of the equipment.

Keywords

antagonism network
antagonistic interactions
community assemblage
interference interactions
local communities
self-organization

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Pérez-Gutiérrez, R. A., López-Ramírez, V., Islas, Á., Alcaraz, L. D., Hernández-González, I., Olivera, B. C. L., Santillán, M., Eguiarte, L. E., Souza, V., Travisano, M., & Olmedo-Alvarez, G. (2013). Antagonism influences assembly of a Bacillus guild in a local community and is depicted as a food-chain network. ISME Journal, 7(3), 487-497. https://doi.org/10.1038/ismej.2012.119

Pérez-Gutiérrez, RA, López-Ramírez, V, Islas, Á, Alcaraz, LD, Hernández-González, I, Olivera, BCL, Santillán, M, Eguiarte, LE, Souza, V, Travisano, M & Olmedo-Alvarez, G 2013, 'Antagonism influences assembly of a Bacillus guild in a local community and is depicted as a food-chain network', ISME Journal, vol. 7, no. 3, pp. 487-497. https://doi.org/10.1038/ismej.2012.119

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Antagonism influences assembly of a Bacillus guild in a local community and is depicted as a food-chain network

Abstract

Bibliographical note

Keywords

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