Antiplatelet and antithrombotic activity of indole-3-carbinol in vitro and in vivo

Min Kyu Park, Yun Hee Rhee, Hyo Jung Lee, Eun Ok Lee, Kwan Hyun Kim, Min Jong Park, Byung Hun Jeon, Bum Sang Shim, Chang Hyun Jung, Seung Hoon Choi, Kyoo Seok Ahn, Sung Hoon Kim

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Indole-3-carbinol, a natural compound found in cruciferous vegetables, is known to have anticancer activity. In the present study, the antiplatelet and antithrombotic activities of indole-3-carbinol were investigated in vitro and in vivo. Indole-3-carbinol significantly inhibited collagen-induced platelet aggregation in human platelet rich plasma (PRP) in a concentration-dependent manner. Indole-3-carbinol significantly inhibited fibrinogen binding to the platelet surface glycoprotein IIb/IIIa (GP IIb/IIIa) receptor by flow cytometric analysis. In addition, the levels of thromboxane B2 (TXB 2) and prostaglandin E2 (PGE2) in collagen stimulated PRP were significantly inhibited in a concentration-dependent manner by indole-3-carbinol. Furthermore, indole-3-carbinol dose-dependently suppressed the death of mice with pulmonary thrombosis induced by intravenous injection of collagen and epinephrine. These results suggest that indole-3-carbinol can be a potent antithrombotic agent with antiplatelet activity through the inhibition of GP IIb/IIIa receptor and thromboxane B2 formation.

Original languageEnglish (US)
Pages (from-to)58-64
Number of pages7
JournalPhytotherapy Research
Volume22
Issue number1
DOIs
StatePublished - Jan 2008

Keywords

  • Antiplatelet activity
  • Antithrombotic agent
  • Glycoprotein IIb/IIIa
  • Indole-3-carbinol
  • Thromboxane B

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