Antiplatelet and antithrombotic activity of indole-3-carbinol in vitro and in vivo

Min Kyu Park; Yun Hee Rhee; Hyo Jung Lee; Eun Ok Lee; Kwan Hyun Kim; Min Jong Park; Byung Hun Jeon; Bum Sang Shim; Chang Hyun Jung; Seung Hoon Choi; Kyoo Seok Ahn; Sung Hoon Kim

doi:10.1002/ptr.2260

Antiplatelet and antithrombotic activity of indole-3-carbinol in vitro and in vivo

Min Kyu Park, Yun Hee Rhee, Hyo Jung Lee, Eun Ok Lee, Kwan Hyun Kim, Min Jong Park, Byung Hun Jeon, Bum Sang Shim, Chang Hyun Jung, Seung Hoon Choi, Kyoo Seok Ahn, Sung Hoon Kim

Research output: Contribution to journal › Article › peer-review

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Abstract

Indole-3-carbinol, a natural compound found in cruciferous vegetables, is known to have anticancer activity. In the present study, the antiplatelet and antithrombotic activities of indole-3-carbinol were investigated in vitro and in vivo. Indole-3-carbinol significantly inhibited collagen-induced platelet aggregation in human platelet rich plasma (PRP) in a concentration-dependent manner. Indole-3-carbinol significantly inhibited fibrinogen binding to the platelet surface glycoprotein IIb/IIIa (GP IIb/IIIa) receptor by flow cytometric analysis. In addition, the levels of thromboxane B₂ (TXB ₂) and prostaglandin E₂ (PGE₂) in collagen stimulated PRP were significantly inhibited in a concentration-dependent manner by indole-3-carbinol. Furthermore, indole-3-carbinol dose-dependently suppressed the death of mice with pulmonary thrombosis induced by intravenous injection of collagen and epinephrine. These results suggest that indole-3-carbinol can be a potent antithrombotic agent with antiplatelet activity through the inhibition of GP IIb/IIIa receptor and thromboxane B₂ formation.

Original language	English (US)
Pages (from-to)	58-64
Number of pages	7
Journal	Phytotherapy Research
Volume	22
Issue number	1
DOIs	https://doi.org/10.1002/ptr.2260
State	Published - Jan 2008
Externally published	Yes

Keywords

Antiplatelet activity
Antithrombotic agent
Glycoprotein IIb/IIIa
Indole-3-carbinol
Thromboxane B

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Antiplatelet and antithrombotic activity of indole-3-carbinol in vitro and in vivo",

abstract = "Indole-3-carbinol, a natural compound found in cruciferous vegetables, is known to have anticancer activity. In the present study, the antiplatelet and antithrombotic activities of indole-3-carbinol were investigated in vitro and in vivo. Indole-3-carbinol significantly inhibited collagen-induced platelet aggregation in human platelet rich plasma (PRP) in a concentration-dependent manner. Indole-3-carbinol significantly inhibited fibrinogen binding to the platelet surface glycoprotein IIb/IIIa (GP IIb/IIIa) receptor by flow cytometric analysis. In addition, the levels of thromboxane B2 (TXB 2) and prostaglandin E2 (PGE2) in collagen stimulated PRP were significantly inhibited in a concentration-dependent manner by indole-3-carbinol. Furthermore, indole-3-carbinol dose-dependently suppressed the death of mice with pulmonary thrombosis induced by intravenous injection of collagen and epinephrine. These results suggest that indole-3-carbinol can be a potent antithrombotic agent with antiplatelet activity through the inhibition of GP IIb/IIIa receptor and thromboxane B2 formation.",

keywords = "Antiplatelet activity, Antithrombotic agent, Glycoprotein IIb/IIIa, Indole-3-carbinol, Thromboxane B",

author = "Park, {Min Kyu} and Rhee, {Yun Hee} and Lee, {Hyo Jung} and Lee, {Eun Ok} and Kim, {Kwan Hyun} and Park, {Min Jong} and Jeon, {Byung Hun} and Shim, {Bum Sang} and Jung, {Chang Hyun} and Choi, {Seung Hoon} and Ahn, {Kyoo Seok} and Kim, {Sung Hoon}",

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AU - Park, Min Kyu

AU - Rhee, Yun Hee

AU - Lee, Hyo Jung

AU - Lee, Eun Ok

AU - Kim, Kwan Hyun

AU - Park, Min Jong

AU - Jeon, Byung Hun

AU - Shim, Bum Sang

AU - Jung, Chang Hyun

AU - Choi, Seung Hoon

AU - Ahn, Kyoo Seok

AU - Kim, Sung Hoon

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AB - Indole-3-carbinol, a natural compound found in cruciferous vegetables, is known to have anticancer activity. In the present study, the antiplatelet and antithrombotic activities of indole-3-carbinol were investigated in vitro and in vivo. Indole-3-carbinol significantly inhibited collagen-induced platelet aggregation in human platelet rich plasma (PRP) in a concentration-dependent manner. Indole-3-carbinol significantly inhibited fibrinogen binding to the platelet surface glycoprotein IIb/IIIa (GP IIb/IIIa) receptor by flow cytometric analysis. In addition, the levels of thromboxane B2 (TXB 2) and prostaglandin E2 (PGE2) in collagen stimulated PRP were significantly inhibited in a concentration-dependent manner by indole-3-carbinol. Furthermore, indole-3-carbinol dose-dependently suppressed the death of mice with pulmonary thrombosis induced by intravenous injection of collagen and epinephrine. These results suggest that indole-3-carbinol can be a potent antithrombotic agent with antiplatelet activity through the inhibition of GP IIb/IIIa receptor and thromboxane B2 formation.

KW - Antiplatelet activity

KW - Antithrombotic agent

KW - Glycoprotein IIb/IIIa

KW - Indole-3-carbinol

KW - Thromboxane B

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Antiplatelet and antithrombotic activity of indole-3-carbinol in vitro and in vivo

Abstract

Keywords

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