asunder is a critical regulator of dynein-dynactin localization during Drosophila spermatogenesis

Michael A. Anderson, Jeanne N. Jodoin, Ethan Lee, Karen G. Hales, Thomas S. Hays, Laura A. Lee

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Spermatogenesis uses mitotic and meiotic cell cycles coordinated with growth and differentiation programs to generate functional sperm. Our analysis of a Drosophila mutant has revealed that asunder (asun), which encodes a conserved protein, is an essential regulator of spermatogenesis. asun spermatocytes arrest during prophase of meiosis I. Strikingly, arrested spermatocytes contain free centrosomes that fail to stably associate with the nucleus. Spermatocytes that overcome arrest exhibit severe defects in meiotic spindle assembly, chromosome segregation, and cytokinesis. Furthermore, the centriole-derived basal body is detached from the nucleus in asun postmeiotic spermatids, resulting in abnormalities later in spermatogenesis. We find that asun spermatocytes and spermatids exhibit drastic reduction of perinuclear dynein-dynactin, a microtubule motor complex. We propose a model in which asun coordinates spermatogenesis by promoting dynein-dynactin recruitment to the nuclear surface, a poorly understood process required for nucleus-centrosome coupling at M phase entry and fidelity of meiotic divisions.

Original languageEnglish (US)
Pages (from-to)2709-2721
Number of pages13
JournalMolecular biology of the cell
Volume20
Issue number11
DOIs
StatePublished - Jun 1 2009

Fingerprint Dive into the research topics of 'asunder is a critical regulator of dynein-dynactin localization during Drosophila spermatogenesis'. Together they form a unique fingerprint.

Cite this