Bacterial cellulose (BC)-based membrane-like biodegradable composites were produced by immersing wet BC pellicles in polyvinyl alcohol (PVA) solution. The BC content in the BC-PVA composites can be adjusted by varying the concentration of PVA solution. Chemical cross-linking of PVA was carried out using glutaraldehyde to increase the mechanical properties of the composites as well as to make the PVA partially to highly water insoluble. Examination by scanning electron microscopy indicated that the PVA not only penetrated the BC network, but also filled the pores within the BC pellicle. Attenuated total reflectance-Fourier transform infrared spectroscopy showed that acetal linkages could be formed in the BC- PVA composites by a cross-linking reaction. Sol-gel results indicated that cross-linking reaction increasingly made PVA insoluble in water resulting in higher gel (crosslinked fraction) content in the PVA. Wide-angle X-ray diffraction results showed decreased crystallinity in crosslinked BC and PVA, as expected. It was also found that crystal size was smaller in PVA after cross-linking. The BC-PVA composites had excellent tensile properties and cross-linking increased these properties further. Thermogravimetric analysis showed higher thermal stability for BC-PVA composites compared to PVA. The cross-linked specimens, especially the highly cross-linked ones, showed even higher thermal stability. The methods developed in this study make it possible to control the PVA content in the composites as well as the cross-linking level of PVA. These composites could be good candidates for replacing traditional non-biodegradable plastics.