Bacterial diterpene synthases: New opportunities for mechanistic enzymology and engineered biosynthesis

Michael J. Smanski, Ryan M. Peterson, Sheng Xiong Huang, Ben Shen

Research output: Contribution to journalReview articlepeer-review

62 Scopus citations


Diterpenoid biosynthesis has been extensively studied in plants and fungi, yet cloning and engineering diterpenoid pathways in these organisms remain challenging. Bacteria are emerging as prolific producers of diterpenoid natural products, and bacterial diterpene synthases are poised to make significant contributions to our understanding of terpenoid biosynthesis. Here we will first survey diterpenoid natural products of bacterial origin and briefly review their biosynthesis with emphasis on diterpene synthases (DTSs) that channel geranylgeranyl diphosphate to various diterpenoid scaffolds. We will then highlight differences of DTSs of bacterial and higher organism origins and discuss the challenges in discovering novel bacterial DTSs. We will conclude by discussing new opportunities for DTS mechanistic enzymology and applications of bacterial DTS in biocatalysis and metabolic pathway engineering.

Original languageEnglish (US)
Pages (from-to)132-141
Number of pages10
JournalCurrent opinion in chemical biology
Issue number1-2
StatePublished - Apr 2012

Fingerprint Dive into the research topics of 'Bacterial diterpene synthases: New opportunities for mechanistic enzymology and engineered biosynthesis'. Together they form a unique fingerprint.

Cite this