Preimplantation embryos of the pig (Days 11 to 15), cow (Days 14 to 16), sheep (Day 14) and pony (Day 16) bind epidermal growth factor (EGF) specifically. Binding was not detected in embryos of the rabbit at Day 5 or 6 or the hamster at Day 3. Transforming growth factor-α displaced [125I] EGF in pig, cow and pony embryos almost as much as unlabeled EGF. The binding affinities of EGF ranged from 12 to 233 pM in pig and cow embryos. The range of species and binding features indicate that the EGF family may play a significant role in mammalian preimplantation development.
Bibliographical noteFunding Information:
This study was supported in part by NJH grant BD 22023t o B.D. Bavister. The sabbatical of B. Fischer in Madison was supported by the Max Kade Foundation, New York, NY. The authors thank D.L. Evenson, R.G. Gottfredson, P.G. GritTin, A.M. Gronowski, D.A. Jensen, L.C. Kotolski, P.S. Lever&al, G. Matbieu, D.L. Northey and T.N. Timm for advice and/or skilled technical assistance.
- epidermal growth factor (EGF)
- preimplantation embryos
- transforming growth factor-α (TGF-α)