Binding of epidermal growth factor and transforming growth factor-α in mammalian preimplantation embryos

B. Fischer; T. A. Rose-Hellekant; L. G. Sheffield; P. J. Bertics; B. D. Bavister

doi:10.1016/0093-691X(94)90503-B

Binding of epidermal growth factor and transforming growth factor-α in mammalian preimplantation embryos

B. Fischer, T. A. Rose-Hellekant, L. G. Sheffield, P. J. Bertics, B. D. Bavister

Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Preimplantation embryos of the pig (Days 11 to 15), cow (Days 14 to 16), sheep (Day 14) and pony (Day 16) bind epidermal growth factor (EGF) specifically. Binding was not detected in embryos of the rabbit at Day 5 or 6 or the hamster at Day 3. Transforming growth factor-α displaced [¹²⁵I] EGF in pig, cow and pony embryos almost as much as unlabeled EGF. The binding affinities of EGF ranged from 12 to 233 pM in pig and cow embryos. The range of species and binding features indicate that the EGF family may play a significant role in mammalian preimplantation development.

Original language	English (US)
Pages (from-to)	879-887
Number of pages	9
Journal	Theriogenology
Volume	41
Issue number	4
DOIs	https://doi.org/10.1016/0093-691X(94)90503-B
State	Published - 1994

Bibliographical note

Funding Information:
This study was supported in part by NJH grant BD 22023t o B.D. Bavister. The sabbatical of B. Fischer in Madison was supported by the Max Kade Foundation, New York, NY. The authors thank D.L. Evenson, R.G. Gottfredson, P.G. GritTin, A.M. Gronowski, D.A. Jensen, L.C. Kotolski, P.S. Lever&al, G. Matbieu, D.L. Northey and T.N. Timm for advice and/or skilled technical assistance.

Keywords

cow
epidermal growth factor (EGF)
pig
preimplantation embryos
transforming growth factor-α (TGF-α)

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abstract = "Preimplantation embryos of the pig (Days 11 to 15), cow (Days 14 to 16), sheep (Day 14) and pony (Day 16) bind epidermal growth factor (EGF) specifically. Binding was not detected in embryos of the rabbit at Day 5 or 6 or the hamster at Day 3. Transforming growth factor-α displaced [125I] EGF in pig, cow and pony embryos almost as much as unlabeled EGF. The binding affinities of EGF ranged from 12 to 233 pM in pig and cow embryos. The range of species and binding features indicate that the EGF family may play a significant role in mammalian preimplantation development.",

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note = "Funding Information: This study was supported in part by NJH grant BD 22023t o B.D. Bavister. The sabbatical of B. Fischer in Madison was supported by the Max Kade Foundation, New York, NY. The authors thank D.L. Evenson, R.G. Gottfredson, P.G. GritTin, A.M. Gronowski, D.A. Jensen, L.C. Kotolski, P.S. Lever&al, G. Matbieu, D.L. Northey and T.N. Timm for advice and/or skilled technical assistance.",

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doi = "10.1016/0093-691X(94)90503-B",

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AU - Rose-Hellekant, T. A.

AU - Sheffield, L. G.

AU - Bertics, P. J.

AU - Bavister, B. D.

N1 - Funding Information: This study was supported in part by NJH grant BD 22023t o B.D. Bavister. The sabbatical of B. Fischer in Madison was supported by the Max Kade Foundation, New York, NY. The authors thank D.L. Evenson, R.G. Gottfredson, P.G. GritTin, A.M. Gronowski, D.A. Jensen, L.C. Kotolski, P.S. Lever&al, G. Matbieu, D.L. Northey and T.N. Timm for advice and/or skilled technical assistance.

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N2 - Preimplantation embryos of the pig (Days 11 to 15), cow (Days 14 to 16), sheep (Day 14) and pony (Day 16) bind epidermal growth factor (EGF) specifically. Binding was not detected in embryos of the rabbit at Day 5 or 6 or the hamster at Day 3. Transforming growth factor-α displaced [125I] EGF in pig, cow and pony embryos almost as much as unlabeled EGF. The binding affinities of EGF ranged from 12 to 233 pM in pig and cow embryos. The range of species and binding features indicate that the EGF family may play a significant role in mammalian preimplantation development.

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KW - transforming growth factor-α (TGF-α)

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Binding of epidermal growth factor and transforming growth factor-α in mammalian preimplantation embryos

Abstract

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Keywords

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