Abstract
Two methods are described for large-scale isolation of islets from the bovine pancreas. With the single-endpoint technique the average number of islets isolated from a 73.3-g pancreas sample was 150,560, representing an estimated total islet volume of 0.391 ml. Pancreases processed by the continuous digestion-filtration method yielded an average of 246,229 islets from a 93.9-g pancreas sample, representing an estimated total islet volume of 0.458 ml. Isolated bovine pancreatic islets demonstrated a well-preserved endocrine cell ultrastructure, rsponded to acute stimulation with propionic acid, and temporarily reversed experimental diabetes in mice.
Original language | English (US) |
---|---|
Pages (from-to) | 206-208 |
Number of pages | 3 |
Journal | Diabetes |
Volume | 38 |
Issue number | SUPPL. 1 |
DOIs | |
State | Published - 1989 |