Bovine islets of Langerhans. Potential source for transplantation?

B. J. Hering, D. Romann, A. Clarius, M. Brendel, M. Slijepcevic, R. G. Bretzel, K. Federlin

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Two methods are described for large-scale isolation of islets from the bovine pancreas. With the single-endpoint technique the average number of islets isolated from a 73.3-g pancreas sample was 150,560, representing an estimated total islet volume of 0.391 ml. Pancreases processed by the continuous digestion-filtration method yielded an average of 246,229 islets from a 93.9-g pancreas sample, representing an estimated total islet volume of 0.458 ml. Isolated bovine pancreatic islets demonstrated a well-preserved endocrine cell ultrastructure, rsponded to acute stimulation with propionic acid, and temporarily reversed experimental diabetes in mice.

Original languageEnglish (US)
Pages (from-to)206-208
Number of pages3
JournalDiabetes
Volume38
Issue numberSUPPL. 1
DOIs
StatePublished - 1989

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