Caenorhabditis elegans mutant allele identification by whole-genome sequencing

Sumeet Sarin; Snehit Prabhu; M. Maggie O'Meara; Itsik Pe'er; Oliver Hobert

doi:10.1038/nmeth.1249

Caenorhabditis elegans mutant allele identification by whole-genome sequencing

Sumeet Sarin, Snehit Prabhu, M. Maggie O'Meara, Itsik Pe'er, Oliver Hobert

Genetics, Cell Biology and Development (CBS)

Research output: Contribution to journal › Article › peer-review

168 Scopus citations

Abstract

Identification of the molecular lesion in Caenorhabditis elegans mutants isolated through forward genetic screens usually involves time-consuming genetic mapping. We used Illumina deep sequencing technology to sequence a complete, mutant C. elegans genome and thus pinpointed a single-nucleotide mutation in the genome that affects a neuronal cell fate decision. This constitutes a proof-of-principle for using whole-genome sequencing to analyze C. elegans mutants.

Original language	English (US)
Pages (from-to)	865-867
Number of pages	3
Journal	Nature Methods
Volume	5
Issue number	10
DOIs	https://doi.org/10.1038/nmeth.1249
State	Published - 2008

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