Caenorhabditis elegans mutant allele identification by whole-genome sequencing

Sumeet Sarin, Snehit Prabhu, M. Maggie O'Meara, Itsik Pe'er, Oliver Hobert

Research output: Contribution to journalArticlepeer-review

168 Scopus citations

Abstract

Identification of the molecular lesion in Caenorhabditis elegans mutants isolated through forward genetic screens usually involves time-consuming genetic mapping. We used Illumina deep sequencing technology to sequence a complete, mutant C. elegans genome and thus pinpointed a single-nucleotide mutation in the genome that affects a neuronal cell fate decision. This constitutes a proof-of-principle for using whole-genome sequencing to analyze C. elegans mutants.

Original languageEnglish (US)
Pages (from-to)865-867
Number of pages3
JournalNature Methods
Volume5
Issue number10
DOIs
StatePublished - 2008

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