Abstract
The separation and detection of biologically active green tea catechins has been accomplished using capillary liquid chromatography/electrospray mass spectrometry (cLC/ESI-MS). Microscale determination (~ 20 ng) of all six catechins in a green tea infusion, and the most extensively studied catechin, (-)epigallocatechin gallate (EGCG), in human plasma is demonstrated by cLC/ESI-MS with selected ion monitoring of protonated molecular ions. The overall quality of the analysis is shown to be dependent on the use of a capillary column with a deactivated, monomeric C18 stationary phase. The high chromatographic separation efficiency of this packed-capillary column, combined with the high sensitivity and selectivity afforded by the mass spectrometer as detector, provide a tellable approach to the analysis of picomolar quantities of these interesting compounds in complex matrices.
Original language | English (US) |
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Pages (from-to) | 1753-1756 |
Number of pages | 4 |
Journal | Rapid Communications in Mass Spectrometry |
Volume | 11 |
Issue number | 16 |
DOIs | |
State | Published - 1997 |