Capsaicin-evoked substance P release in rat dorsal horn increases after peripheral inflammation: A microdialysis study

Abdullahi Warsame Afrah; Henrik Gustafsson; Leif Olgart; Ernst Brodin; Carl Olav Stiller; Bradley K. Taylor

doi:10.1016/j.neulet.2004.07.041

Capsaicin-evoked substance P release in rat dorsal horn increases after peripheral inflammation: A microdialysis study

Abdullahi Warsame Afrah, Henrik Gustafsson, Leif Olgart, Ernst Brodin, Carl Olav Stiller, Bradley K. Taylor

Neuroscience

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Numerous in vitro studies suggest that inflammation is associated with enhanced release of substance P (SP) in the dorsal horn. To test the hypothesis that inflammation increases the evoked concentration of SP in the intact animal, we used in vivo microdialysis with a highly sensitive radioimmunoassay to monitor SP-like immunoreactivity (SP-LI) in the dorsal horn. Seven days after the induction of persistent unilateral inflammation with hindpaw injection of complete Freund's adjuvant, perfusion of the microdialysis probe with 10 μM capsaicin (a concentration which failed to induce SP-LI release in rats without inflammation) induced a significant increase of microdialysate SP-LI. Inclusion of an NMDA antagonist in the perfusion fluid completely blocked this capsaicin-evoked SP release. Administration of a five-fold higher dose of capsaicin did not further increase SP release. These results in a rat model of chronic arthritis suggest that persistent inflammatory signaling facilitates capsaicin-evoked SP release in the dorsal horn in vivo.

Original language	English (US)
Pages (from-to)	226-230
Number of pages	5
Journal	Neuroscience Letters
Volume	368
Issue number	2
DOIs	https://doi.org/10.1016/j.neulet.2004.07.041
State	Published - Sep 23 2004

Bibliographical note

Funding Information:
The study was supported by NIH grants DA10356 and NS45954 to B.K.T., the Swedish Medical Research Council 6836, Magnus Bergvalls Stiftelse, the Swedish Society of Medicine, the Karolinska Institute and Tulane University. We thank Annika Olsson for excellent technical assistance with the RIAs.

Keywords

Capsaicin
Inflammation
Microdialysis
NMDA
Pain
Spinal cord
Substance P

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title = "Capsaicin-evoked substance P release in rat dorsal horn increases after peripheral inflammation: A microdialysis study",

abstract = "Numerous in vitro studies suggest that inflammation is associated with enhanced release of substance P (SP) in the dorsal horn. To test the hypothesis that inflammation increases the evoked concentration of SP in the intact animal, we used in vivo microdialysis with a highly sensitive radioimmunoassay to monitor SP-like immunoreactivity (SP-LI) in the dorsal horn. Seven days after the induction of persistent unilateral inflammation with hindpaw injection of complete Freund's adjuvant, perfusion of the microdialysis probe with 10 μM capsaicin (a concentration which failed to induce SP-LI release in rats without inflammation) induced a significant increase of microdialysate SP-LI. Inclusion of an NMDA antagonist in the perfusion fluid completely blocked this capsaicin-evoked SP release. Administration of a five-fold higher dose of capsaicin did not further increase SP release. These results in a rat model of chronic arthritis suggest that persistent inflammatory signaling facilitates capsaicin-evoked SP release in the dorsal horn in vivo.",

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AU - Brodin, Ernst

AU - Stiller, Carl Olav

AU - Taylor, Bradley K.

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Capsaicin-evoked substance P release in rat dorsal horn increases after peripheral inflammation: A microdialysis study

Abstract

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Keywords

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