A low-cost, label-free, ultra-sensitive electric immunoassay is developed for the detection of swine influenza virus (SIV) H1N1. The assay is based on the excellent electrical properties of single-walled carbon nanotubes (SWCNTs). Antibody-virus complexes influence the conductance of underlying SWCNT thin film, which has been constructed by facile layer-by-layer self-assembly. The basic steps of conventional immunoassay are performed followed by the electric characterization of immunochips at the last stage. The resistance of immunochips tends to increase upon surface adsorption of macromolecules such as poly-l-lysine, anti-SIV antibodies, and SIVs during the assay. The resistance shift after the binding of SIV with anti-SIV antibody is normalized with the resistances of bare devices. The sensor selectivity tests are performed with non-SIVs, showing the normalized resistance shift of 12% as a background. The detection limit of 180 TCID50/ml of SIV is obtained suggesting a potential application of this assay as point-of-care detection or monitoring system. This facile CNT-based immunoassay also has the potential to be used as a sensing platform for lab-on-a-chip system.
Bibliographical noteFunding Information:
The authors acknowledge the DARPA M/NEMS Science and Technology Fundamentals Research Program for funding support through the Micro/Nano Fluidics Fundamentals Focus (MF3) Center. A part of this work was conducted at the Nanofabrication Center and Characterization Facility at the University of Minnesota, which are partially supported by NSF through the NNIN program.
Copyright 2011 Elsevier B.V., All rights reserved.
- Carbon nanotube
- Swine influenza virus H1N1
- Virus sensor