CD133 initiates tumors, induces epithelial-mesenchymal transition and increases metastasis in pancreatic cancer

Alice Nomura; Sulagna Banerjee; Rohit Chugh; Vikas Dudeja; Masato Yamamoto; Selwyn M. Vickers; Ashok K. Saluja

doi:10.18632/oncotarget.3228

CD133 initiates tumors, induces epithelial-mesenchymal transition and increases metastasis in pancreatic cancer

Alice Nomura, Sulagna Banerjee, Rohit Chugh, Vikas Dudeja, Masato Yamamoto, Selwyn M. Vickers, Ashok K. Saluja

Surgery

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93 Scopus citations

Abstract

CD133 has been implicated as a cancer stem cell (CSC) surface marker in several malignancies including pancreatic cancer. However, the functional role of this surface glycoprotein in the cancer stem cell remains elusive. In this study, we determined that CD133 overexpression induced "stemness" properties in MIA-PaCa2 cells along with increased tumorigenicity, tumor progression, and metastasis in vivo. Additionally, CD133 expression induced epithelial-mesenchymal transition (EMT) and increased in vitro invasion. EMT induction and increased invasiveness were mediated by NF-κB activation, as inhibition of NF-κB mitigated these effects. This study showed that CD133 expression contributes to pancreatic cancer "stemness," tumorigenicity, EMT induction, invasion, and metastasis.

Original language	English (US)
Pages (from-to)	8313-8322
Number of pages	10
Journal	Oncotarget
Volume	6
Issue number	10
DOIs	https://doi.org/10.18632/oncotarget.3228
State	Published - 2015

Keywords

CD133
Invasion
Metastasis
NF-κB
Pancreatic Cancer

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.18632/oncotarget.3228

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T1 - CD133 initiates tumors, induces epithelial-mesenchymal transition and increases metastasis in pancreatic cancer

AU - Nomura, Alice

AU - Banerjee, Sulagna

AU - Chugh, Rohit

AU - Dudeja, Vikas

AU - Yamamoto, Masato

AU - Vickers, Selwyn M.

AU - Saluja, Ashok K.

PY - 2015

Y1 - 2015

N2 - CD133 has been implicated as a cancer stem cell (CSC) surface marker in several malignancies including pancreatic cancer. However, the functional role of this surface glycoprotein in the cancer stem cell remains elusive. In this study, we determined that CD133 overexpression induced "stemness" properties in MIA-PaCa2 cells along with increased tumorigenicity, tumor progression, and metastasis in vivo. Additionally, CD133 expression induced epithelial-mesenchymal transition (EMT) and increased in vitro invasion. EMT induction and increased invasiveness were mediated by NF-κB activation, as inhibition of NF-κB mitigated these effects. This study showed that CD133 expression contributes to pancreatic cancer "stemness," tumorigenicity, EMT induction, invasion, and metastasis.

AB - CD133 has been implicated as a cancer stem cell (CSC) surface marker in several malignancies including pancreatic cancer. However, the functional role of this surface glycoprotein in the cancer stem cell remains elusive. In this study, we determined that CD133 overexpression induced "stemness" properties in MIA-PaCa2 cells along with increased tumorigenicity, tumor progression, and metastasis in vivo. Additionally, CD133 expression induced epithelial-mesenchymal transition (EMT) and increased in vitro invasion. EMT induction and increased invasiveness were mediated by NF-κB activation, as inhibition of NF-κB mitigated these effects. This study showed that CD133 expression contributes to pancreatic cancer "stemness," tumorigenicity, EMT induction, invasion, and metastasis.

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KW - NF-κB

KW - Pancreatic Cancer

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CD133 initiates tumors, induces epithelial-mesenchymal transition and increases metastasis in pancreatic cancer

Abstract

Keywords

UN SDGs

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