TY - JOUR
T1 - Cell-cycle analysis in phagotrophic microorganisms from flow cytometric histograms
AU - Hatzis, Christos
AU - Fredrickson, A. G.
AU - Srienc, Friedrich
N1 - Funding Information:
This work was supported by the National Science Foundation\ Grant Nos[ NSF:BCS 7508288 and 8990984[
PY - 1997/5/21
Y1 - 1997/5/21
N2 - The inability of certain phagotrophic protozoa to phagocytose right before and right after cell division was utilized to measure the distributions of newborn and dividing cells and obtain information on the kinetics of the cell cycle. A fluorescent microsphere labeling assay was used in conjunction with flow cytometry to identify the non-feeding cells and measure their DNA and protein distributions. These distributions, which are typically bimodal, were decomposed to the two component distributions by means of a new algorithm, which is based on the assumption that cells partition their DNA and the remaining cellular material evenly at division. The estimated population fractions in the two non-feeding phases before and after division and the corresponding DNA and protein distributions provide significant information on the kinetics of the cell cycle. The transient labeling experiments revealed a significant variability in the timing of the pre-division non-feeding phase with the smaller cells of this subpopulation spending more time in preparation for division. From the determined distributions of newborn and dividing cells, the single-cell growth rates and division probabilities were also calculated. This type of analysis provides essential information for the development of corpuscular, multi-staged cell population models.
AB - The inability of certain phagotrophic protozoa to phagocytose right before and right after cell division was utilized to measure the distributions of newborn and dividing cells and obtain information on the kinetics of the cell cycle. A fluorescent microsphere labeling assay was used in conjunction with flow cytometry to identify the non-feeding cells and measure their DNA and protein distributions. These distributions, which are typically bimodal, were decomposed to the two component distributions by means of a new algorithm, which is based on the assumption that cells partition their DNA and the remaining cellular material evenly at division. The estimated population fractions in the two non-feeding phases before and after division and the corresponding DNA and protein distributions provide significant information on the kinetics of the cell cycle. The transient labeling experiments revealed a significant variability in the timing of the pre-division non-feeding phase with the smaller cells of this subpopulation spending more time in preparation for division. From the determined distributions of newborn and dividing cells, the single-cell growth rates and division probabilities were also calculated. This type of analysis provides essential information for the development of corpuscular, multi-staged cell population models.
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U2 - 10.1006/jtbi.1996.0348
DO - 10.1006/jtbi.1996.0348
M3 - Article
AN - SCOPUS:0031582386
SN - 0022-5193
VL - 186
SP - 131
EP - 144
JO - Journal of Theoretical Biology
JF - Journal of Theoretical Biology
IS - 2
ER -