TY - JOUR
T1 - Characteristics of vero cytotoxin producing Escherichia coli associated with intestinal colonization and diarrhea in calves
AU - Richard Dorn, C.
AU - Francis, David H.
AU - Angrick, Elisabeth J.
AU - Willgohs, Jo Ann A.
AU - Wilson, Richard A.
AU - Collins, James E.
AU - Jenke, Bruce H.
AU - Shawd, Stacey J.
PY - 1993/7
Y1 - 1993/7
N2 - Isolates of Escherichia coli which produce Vero cytotoxin (VTEC) were obtained during 1983-1989 from calves raised in 5 north-central states of the USA. All of the calves experienced intestinal epithelial colonization by VTEC, diarrhea or both; twelve of the calves had bloody diarrhea. Twenty one isolates were serogroup O111 and the others were O103, O69, O45, O26, O5, or non-typable (4 isolates). All but one of the isolates hybridized with the CVD419 probe which identifies most VTEC strains. Thirty two isolates hybridized with the VT1 probe, 3 with both the VT1 and VT2 probes, and one with neither probe. The culture filtrate of the VT probe negative isolate was partially neutralized by SLT I monoclonal antibody. For the other isolates, the results of toxin neutralization by anti-SLT I and anti-SLT II monoclonal antibodies corresponded exactly with the VT1 and VT2 probe hybridization results. Three of the strains adhered in a localized manner to HEp-2 cells and Intestine 407 cells.
AB - Isolates of Escherichia coli which produce Vero cytotoxin (VTEC) were obtained during 1983-1989 from calves raised in 5 north-central states of the USA. All of the calves experienced intestinal epithelial colonization by VTEC, diarrhea or both; twelve of the calves had bloody diarrhea. Twenty one isolates were serogroup O111 and the others were O103, O69, O45, O26, O5, or non-typable (4 isolates). All but one of the isolates hybridized with the CVD419 probe which identifies most VTEC strains. Thirty two isolates hybridized with the VT1 probe, 3 with both the VT1 and VT2 probes, and one with neither probe. The culture filtrate of the VT probe negative isolate was partially neutralized by SLT I monoclonal antibody. For the other isolates, the results of toxin neutralization by anti-SLT I and anti-SLT II monoclonal antibodies corresponded exactly with the VT1 and VT2 probe hybridization results. Three of the strains adhered in a localized manner to HEp-2 cells and Intestine 407 cells.
UR - http://www.scopus.com/inward/record.url?scp=0027177598&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027177598&partnerID=8YFLogxK
U2 - 10.1016/0378-1135(93)90136-U
DO - 10.1016/0378-1135(93)90136-U
M3 - Article
C2 - 8236776
AN - SCOPUS:0027177598
SN - 0378-1135
VL - 36
SP - 149
EP - 159
JO - Veterinary Microbiology
JF - Veterinary Microbiology
IS - 1-2
ER -