Characterization of a chimeric foot-and-mouth disease virus bearing a bovine rhinitis B virus leader proteinase

Sabena Uddowla; Juan M. Pacheco; Christopher Larson; Elizabeth Bishop; Luis L. Rodriguez; Devendra K. Rai; Jonathan Arzt; Elizabeth Rieder

doi:10.1016/j.virol.2013.08.035

Characterization of a chimeric foot-and-mouth disease virus bearing a bovine rhinitis B virus leader proteinase

Sabena Uddowla, Juan M. Pacheco, Christopher Larson, Elizabeth Bishop, Luis L. Rodriguez, Devendra K. Rai, Jonathan Arzt, Elizabeth Rieder

Veterinary Population Medicine

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Bovine rhinitis B virus (BRBV) shares many motifs and sequence similarities with foot-and-mouth disease virus (FMDV). This study examined if the BRBV leader proteinase (L^pro ) could functionally replace that of FMDV. A mutant A₂₄L_BRV3D_YR FMDV engineered with the BRBV L^pro and an antigenic marker in the 3D polymerase exhibited growth properties and eIF4G cleavage similar to parental A₂₄WT virus. The A₂₄L_BRV3D_YR type I interferon activity in infected bovine cells resembled that of A₂₄LL virus that lacks L^pro, but this effect was less pronounced for A₂₄L_BRV3D_YR infected porcine cells. In vivo studies showed that the A₂₄L_BRV3D_YR virus was attenuated in cattle, and exhibited low virulence in pigs exposed by direct contact. The mutant virus induced protective immunity in cattle against challenge with parental A₂₄WT. These results provide evidence that L^pro of different Aphthoviruses are not fully functionally interchangeable and have roles that may depend on the nature of the infected host.

Original language	English (US)
Pages (from-to)	172-180
Number of pages	9
Journal	Virology
Volume	447
Issue number	1-2
DOIs	https://doi.org/10.1016/j.virol.2013.08.035
State	Published - Dec 2013

Bibliographical note

Funding Information:
We thank Dr. Alfonso Clavijo for the gift of mAbs against the FMDV 3D pol and Drs. Marvin Grubman and Paul Lawrence for fruitful discussions. This research was supported by CRIS Project no. 1940-32000-053-00D , 1940-32000-057-00D , Agricultural Research Service (ARS), U.S. Department of Agriculture (Dr. Elizabeth Rieder). Dr. Sabena Uddowla, Dr. Devendra K. Rai and Mr. Christopher Larson were the recipient of a fellowship by the Plum Island Animal Disease Research Participation Program administered by the Oak Ridge Institute for Science and Education through an interagency agreement between the U.S. Department of Energy and the U.S. Department of Agriculture.

Keywords

Bovine rhinitis B virus (BRBV) leader proteinase
Chimeric foot-and-mouth disease virus (FMDV)
FMDV pathogenesis

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title = "Characterization of a chimeric foot-and-mouth disease virus bearing a bovine rhinitis B virus leader proteinase",

abstract = "Bovine rhinitis B virus (BRBV) shares many motifs and sequence similarities with foot-and-mouth disease virus (FMDV). This study examined if the BRBV leader proteinase (Lpro ) could functionally replace that of FMDV. A mutant A24LBRV3DYR FMDV engineered with the BRBV Lpro and an antigenic marker in the 3D polymerase exhibited growth properties and eIF4G cleavage similar to parental A24WT virus. The A24LBRV3DYR type I interferon activity in infected bovine cells resembled that of A24LL virus that lacks Lpro, but this effect was less pronounced for A24LBRV3DYR infected porcine cells. In vivo studies showed that the A24LBRV3DYR virus was attenuated in cattle, and exhibited low virulence in pigs exposed by direct contact. The mutant virus induced protective immunity in cattle against challenge with parental A24WT. These results provide evidence that Lpro of different Aphthoviruses are not fully functionally interchangeable and have roles that may depend on the nature of the infected host.",

keywords = "Bovine rhinitis B virus (BRBV) leader proteinase, Chimeric foot-and-mouth disease virus (FMDV), FMDV pathogenesis",

author = "Sabena Uddowla and Pacheco, {Juan M.} and Christopher Larson and Elizabeth Bishop and Rodriguez, {Luis L.} and Rai, {Devendra K.} and Jonathan Arzt and Elizabeth Rieder",

note = "Funding Information: We thank Dr. Alfonso Clavijo for the gift of mAbs against the FMDV 3D pol and Drs. Marvin Grubman and Paul Lawrence for fruitful discussions. This research was supported by CRIS Project no. 1940-32000-053-00D , 1940-32000-057-00D , Agricultural Research Service (ARS), U.S. Department of Agriculture (Dr. Elizabeth Rieder). Dr. Sabena Uddowla, Dr. Devendra K. Rai and Mr. Christopher Larson were the recipient of a fellowship by the Plum Island Animal Disease Research Participation Program administered by the Oak Ridge Institute for Science and Education through an interagency agreement between the U.S. Department of Energy and the U.S. Department of Agriculture.",

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AU - Rodriguez, Luis L.

AU - Rai, Devendra K.

AU - Arzt, Jonathan

AU - Rieder, Elizabeth

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Characterization of a chimeric foot-and-mouth disease virus bearing a bovine rhinitis B virus leader proteinase

Abstract

Bibliographical note

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