Characterization of a flatworm inositol (1,4,5) trisphosphate receptor (IP3R) reveals a role in reproductive physiology

Dan Zhang; Xiaolong Liu; John D. Chan; Jonathan S. Marchant

doi:10.1016/j.ceca.2013.01.003

Characterization of a flatworm inositol (1,4,5) trisphosphate receptor (IP₃R) reveals a role in reproductive physiology

Dan Zhang, Xiaolong Liu, John D. Chan, Jonathan S. Marchant

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Inositol 1,4,5-trisphosphate receptors (IP₃Rs) are intracellular Ca²⁺ channels that elevate cytoplasmic Ca²⁺ in response to the second messenger IP₃. Here, we describe the identification and in vivo functional characterization of the planarian IP₃R, the first intracellular Ca²⁺ channel to be defined in flatworms. A single IP₃R gene in Dugesia japonica encoded a 2666 amino acid protein (Dj.IP₃R) that shared well conserved structural features with vertebrate IP₃R counterparts. Expression of an NH₂-terminal Dj.IP₃R region (amino acid residues 223-585) recovered high affinity ³H-IP₃ binding (0.9±0.1nM) which was abolished by a single point mutation of an arginine residue (R495L) important for IP₃ coordination. In situ hybridization revealed that Dj.IP₃R mRNA was most strongly expressed in the pharynx and optical nerve system as well as the reproductive system in sexualized planarians. Consistent with this observed tissue distribution, in vivo RNAi of Dj.IP₃R resulted in a decreased egg-laying behavior suggesting Dj.IP₃R plays an upstream role in planarian reproductive physiology.

Original language	English (US)
Pages (from-to)	307-314
Number of pages	8
Journal	Cell Calcium
Volume	53
Issue number	5-6
DOIs	https://doi.org/10.1016/j.ceca.2013.01.003
State	Published - May 2013

Bibliographical note

Funding Information:
Work in the laboratory was supported by the NIH ( GM088790 to J.S.M.) and NSF (MCB 0919933 ). J.D.C. was supported by a Stem Cell Biology Training Grant ( T32HD060536 ). Appendix A

Keywords

Invertebrate
Planarian
Platyhelminth

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title = "Characterization of a flatworm inositol (1,4,5) trisphosphate receptor (IP3R) reveals a role in reproductive physiology",

abstract = "Inositol 1,4,5-trisphosphate receptors (IP3Rs) are intracellular Ca2+ channels that elevate cytoplasmic Ca2+ in response to the second messenger IP3. Here, we describe the identification and in vivo functional characterization of the planarian IP3R, the first intracellular Ca2+ channel to be defined in flatworms. A single IP3R gene in Dugesia japonica encoded a 2666 amino acid protein (Dj.IP3R) that shared well conserved structural features with vertebrate IP3R counterparts. Expression of an NH2-terminal Dj.IP3R region (amino acid residues 223-585) recovered high affinity 3H-IP3 binding (0.9±0.1nM) which was abolished by a single point mutation of an arginine residue (R495L) important for IP3 coordination. In situ hybridization revealed that Dj.IP3R mRNA was most strongly expressed in the pharynx and optical nerve system as well as the reproductive system in sexualized planarians. Consistent with this observed tissue distribution, in vivo RNAi of Dj.IP3R resulted in a decreased egg-laying behavior suggesting Dj.IP3R plays an upstream role in planarian reproductive physiology.",

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author = "Dan Zhang and Xiaolong Liu and Chan, {John D.} and Marchant, {Jonathan S.}",

note = "Funding Information: Work in the laboratory was supported by the NIH ( GM088790 to J.S.M.) and NSF (MCB 0919933 ). J.D.C. was supported by a Stem Cell Biology Training Grant ( T32HD060536 ). Appendix A ",

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