Swine, unlike other artiodactyls, but similar to humans, utilize both lambda and kappa light chain isotypes almost equally in the generation of their antibody repertoire. The porcine antibody light chain loci have previously been characterized in a single Duroc sow in which was seen extensive allelic variation between light chain genes on homologous chromosomes. However, the extent of variation between individuals is completely unknown. Using deep sequencing of cDNA-derived amplicons from five pigs, we report the identification and characterization of an IGLV gene that is functional and highly expressed in some animals, yet completely absent in others. Our findings provide a possible rationale for the known individual-to-individual variation in antibody responses to vaccination, infectious challenge, and subsequent disease outcome.
Bibliographical noteFunding Information:
Acknowledgments We thank Juan Abrahante for assistance and advice in sequence data management. Funding was provided by the National Pork Board grant 10-139 (J.C.S. and M.P.M.). J.C.S. was supported by the Molecular Virology Training Grant T32 AI83196 from the National Institutes of Health and a Doctoral Dissertation Fellowship from the University of Minnesota.
- Light chain
- Sus scrofa