TY - JOUR
T1 - Characterization of Genetic Resistance to Fusarium Head Blight and Bacterial Leaf Streak in Intermediate Wheatgrass (Thinopyrum intermedium)
AU - Bajgain, Prabin
AU - Zhang, Xiaofei
AU - Turner, M. Kathryn
AU - Curland, Rebecca D.
AU - Heim, Brett
AU - Dill-Macky, Ruth
AU - Ishimaru, Carol A.
AU - Anderson, James A.
N1 - Publisher Copyright:
© 2019 by the authors.
PY - 2019/8/3
Y1 - 2019/8/3
N2 - Intermediate wheatgrass (IWG, Thinopyrum intermedium, (Host) Barkworth & D.R. Dewey subsp. intermedium, 2n = 6x = 42) is a novel perennial crop currently undergoing domestication efforts. It offers remarkable ecosystem services and yields higher relative to other perennial grain crops. While IWG is mostly resistant to Fusarium head blight (FHB), identifying genomic regions associated with resistance will help protect the crop from potential disease epidemics. An IWG biparental population of 108 individuals was developed by crossing parents differing in their response to FHB and bacterial leaf streak (BLS). The population was screened for disease reaction over three years using isolates collected from IWG plants in St. Paul, Minnesota, USA. Linkage maps representing the 21 IWG chromosomes were constructed from 4622 Single Nucleotide Polymorphism (SNP) markers, with one SNP at every 0.74 cM. Interval mapping identified 15 quantitative trait loci (QTL) associated with FHB resistance and 11 with BLS resistance. Models with two or three QTL combinations reduced FHB disease severity by up to 15%, and BLS by up to 17%. When markers associated with FHB resistance were used as cofactors in genomic selection models, trait predictive ability improved by 24–125%. These genomic regions and genetic markers associated with FHB and BLS resistance can also be used to safeguard annual cereal grains through gene introgression and selective breeding.
AB - Intermediate wheatgrass (IWG, Thinopyrum intermedium, (Host) Barkworth & D.R. Dewey subsp. intermedium, 2n = 6x = 42) is a novel perennial crop currently undergoing domestication efforts. It offers remarkable ecosystem services and yields higher relative to other perennial grain crops. While IWG is mostly resistant to Fusarium head blight (FHB), identifying genomic regions associated with resistance will help protect the crop from potential disease epidemics. An IWG biparental population of 108 individuals was developed by crossing parents differing in their response to FHB and bacterial leaf streak (BLS). The population was screened for disease reaction over three years using isolates collected from IWG plants in St. Paul, Minnesota, USA. Linkage maps representing the 21 IWG chromosomes were constructed from 4622 Single Nucleotide Polymorphism (SNP) markers, with one SNP at every 0.74 cM. Interval mapping identified 15 quantitative trait loci (QTL) associated with FHB resistance and 11 with BLS resistance. Models with two or three QTL combinations reduced FHB disease severity by up to 15%, and BLS by up to 17%. When markers associated with FHB resistance were used as cofactors in genomic selection models, trait predictive ability improved by 24–125%. These genomic regions and genetic markers associated with FHB and BLS resistance can also be used to safeguard annual cereal grains through gene introgression and selective breeding.
KW - Bacterial leaf streak
KW - Crop domestication
KW - Disease resistance mapping
KW - Fusarium head blight
KW - Intermediate wheatgrass
KW - QTL
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U2 - 10.3390/agronomy9080429
DO - 10.3390/agronomy9080429
M3 - Article
AN - SCOPUS:85070194065
SN - 2073-4395
VL - 9
JO - Agronomy
JF - Agronomy
IS - 8
M1 - 429
ER -