Characterization of the PEG layer of sterically stabilized liposomes: A SAXS study

Z. Varga; A. Wacha; U. Vainio; J. Gummel; A. Bóta

doi:10.1016/j.chemphyslip.2011.12.011

Characterization of the PEG layer of sterically stabilized liposomes: A SAXS study

Z. Varga, A. Wacha, U. Vainio, J. Gummel, A. Bóta

Chemistry (Twin Cities)

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

Synchrotron small-angle X-ray scattering analysis of the bilayer structure of a pharmacologically relevant sterically stabilized liposome system is presented. Describing the electron density profile of the bilayer with the superposition of Gaussian functions, the contribution of the poly(ethylene glycol) (PEG) layers to the total electron density was identified. The changes in the thickness of the PEG layer as well as the distribution of the PEG chains among the outer and inner leaflets of the bilayers were followed by changing the molar ratio of the PEG-lipid and the molar weight of the PEG molecule.

Original language	English (US)
Pages (from-to)	387-392
Number of pages	6
Journal	Chemistry and Physics of Lipids
Volume	165
Issue number	4
DOIs	https://doi.org/10.1016/j.chemphyslip.2011.12.011
State	Published - May 2012

Bibliographical note

Funding Information:
This work was supported by the Hungarian Scientific Research Fund (OTKA, Hungary) and the National Innovation Office (NIH, Hungary) under grant agreement CNK-81056 . The research leading to these results has received funding from the European Community's Seventh Framework Programme (FP7/2007–2013) under grant agreement no 226716 at DESY/HASYLAB. We are grateful to the European Synchrotron Radiation Facility for provision of beam time at ID02 (proposal no. SC-3269).

Keywords

Liposomes
Poly(ethylene glycol)
Small-angle X-ray scattering
Sterical stabilization
Structural characterization

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title = "Characterization of the PEG layer of sterically stabilized liposomes: A SAXS study",

abstract = "Synchrotron small-angle X-ray scattering analysis of the bilayer structure of a pharmacologically relevant sterically stabilized liposome system is presented. Describing the electron density profile of the bilayer with the superposition of Gaussian functions, the contribution of the poly(ethylene glycol) (PEG) layers to the total electron density was identified. The changes in the thickness of the PEG layer as well as the distribution of the PEG chains among the outer and inner leaflets of the bilayers were followed by changing the molar ratio of the PEG-lipid and the molar weight of the PEG molecule.",

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author = "Z. Varga and A. Wacha and U. Vainio and J. Gummel and A. B{\'o}ta",

note = "Funding Information: This work was supported by the Hungarian Scientific Research Fund (OTKA, Hungary) and the National Innovation Office (NIH, Hungary) under grant agreement CNK-81056 . The research leading to these results has received funding from the European Community's Seventh Framework Programme (FP7/2007–2013) under grant agreement no 226716 at DESY/HASYLAB. We are grateful to the European Synchrotron Radiation Facility for provision of beam time at ID02 (proposal no. SC-3269).",

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AU - Vainio, U.

AU - Gummel, J.

AU - Bóta, A.

N1 - Funding Information: This work was supported by the Hungarian Scientific Research Fund (OTKA, Hungary) and the National Innovation Office (NIH, Hungary) under grant agreement CNK-81056 . The research leading to these results has received funding from the European Community's Seventh Framework Programme (FP7/2007–2013) under grant agreement no 226716 at DESY/HASYLAB. We are grateful to the European Synchrotron Radiation Facility for provision of beam time at ID02 (proposal no. SC-3269).

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AB - Synchrotron small-angle X-ray scattering analysis of the bilayer structure of a pharmacologically relevant sterically stabilized liposome system is presented. Describing the electron density profile of the bilayer with the superposition of Gaussian functions, the contribution of the poly(ethylene glycol) (PEG) layers to the total electron density was identified. The changes in the thickness of the PEG layer as well as the distribution of the PEG chains among the outer and inner leaflets of the bilayers were followed by changing the molar ratio of the PEG-lipid and the molar weight of the PEG molecule.

KW - Liposomes

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KW - Small-angle X-ray scattering

KW - Sterical stabilization

KW - Structural characterization

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Characterization of the PEG layer of sterically stabilized liposomes: A SAXS study

Abstract

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Keywords

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