Characterization of three porcine reproductive and respiratory syndrome virus isolates from a single swine farm bearing strong homology to a vaccine strain

Yi feng Jiang, Tian qi Xia, Yan jun Zhou, Ling xue Yu, Shen Yang, Qin feng Huang, Li wei Li, Fei Gao, Ze hui Qu, Wu Tong, Guang zhi Tong

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

Three porcine reproductive and respiratory syndrome viruses (PRRSV), NT1, NT2, and NT3, were isolated from three dying piglets from a single pig farm in Jiangsu Province, China. Whole genome sequencing revealed that the three isolates share the highest homology with JXA1-P80, an attenuated vaccine strain developed by serial passage of highly pathogenic PRRSV JXA1 in MARC-145 cells. More than ten amino acids residues in ORF1a, ORF1b, GP4, and GP5 that were thought to be unique to JXA1 attenuated on MARC-145 cells were each found in the corresponding locations of NT1, NT2, and NT3. In virulence assays, piglets infected with NT1, NT2, or NT3 exhibited clinical signs of disease, including high fever, anorexia, and respiratory distress, leading to the death of the majority of the piglets within two weeks. Collectively, these data indicate that NT1, NT2, and NT3 are highly pathogenic PRRSVs and they are likely to be revertants of the vaccine strain JXA1-P80.

Original languageEnglish (US)
Pages (from-to)242-249
Number of pages8
JournalVeterinary Microbiology
Volume179
Issue number3-4
DOIs
StatePublished - Sep 30 2015

Bibliographical note

Funding Information:
This study was supported by Natural Science Foundation of China (grant no. 31302098); National Basic Research Program of China (973 Plan, grant no. 2014CB542700)

Keywords

  • Genetic diversity
  • JXA1-P80
  • PRRSV
  • Virulence reversion

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