Comparative analysis of 18 sex pheromone plasmids from Enterococcus faecalis: Detection of a new insertion element on pPD1 and implications for the evolution of this plasmid family

Helmut Hirt, Reinhard Wirth, Albrecht Muscholl

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

A new IS element, IS1062, related to the enterococcal IS elements IS6770 and IS1252, was detected in the 3'-terminus of the surface exclusion gene, sep1, of sex pheromone plasmid pPD1 in Enterococcus faecalis. pPD1-bearing cells lack the surface exclusion function, probably as a consequence of this insertion. Analysis of pAD1 and pPD1 sequences (7.5 kb and 2.7 kb, respectively) downstream of their aggregation substance genes revealed no similarity in these DNA regions. Detailed DNA/DNA hybridization studies using DNA probes specific for various pAD1-encoded genes needed for plasmid transfer indicated that the sex pheromone plasmids have evolved by repeated recombination and insertion of diverse transposable elements which presumably account for recent acquisition of antibiotic resistances.

Original languageEnglish (US)
Pages (from-to)640-647
Number of pages8
JournalMolecular and General Genetics
Volume252
Issue number6
DOIs
StatePublished - Nov 21 1996
Externally publishedYes

Keywords

  • Enterococcus faecalis
  • Insertion element IS1062
  • Plasmid evolution
  • Sex pheromone plasmids

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