Comparative studies of different stromal cell microenvironments in support of human B-cell development

Daitaro Kurosaka; Tucker W. Lebien; Julie A.R. Pribyl

doi:10.1016/S0301-472X(99)00067-3

Comparative studies of different stromal cell microenvironments in support of human B-cell development

Daitaro Kurosaka, Tucker W. Lebien, Julie A.R. Pribyl

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

This study compared human murine stromal cells for their capacity to support human hematopoietic stem cell (HSC) development into the B lineage. FACS sorted human fetal bone marrow (BM) HSC (CD34⁺ CD19^- or CD34⁺/CD10^- /CD19^-/CD45RA^-) were cultured on human fetal BM stromal cells, human skin fibroblasts, or murine S17 stromal cells and analyzed by flow cytometry or reverse transcriptase polymerase chain reaction. CD34⁺CD19^- HSC on human BM stromal cells or fibroblasts differentiated into B-lineage cells with a continuum in density of surface CD19 expression, and some cells expressing μ/κ or μ/λ B-cell receptors. In contrast, CD19⁺ cells from S17 cultures had two- to fourfold higher levels of CD19, but no cells expressing B-cell receptors. The number and percentage of CD19⁺ cells was high, intermediate, or low in the human BM, human fibroblast, or murine S17 stromal cell cultures, respectively. Reverse transcriptase polymerase chain reaction analysis showed that TdT, CD19, and D(H)Q52- J(H) rearrangements were expressed at comparable levels when CD34⁺/CD19^- HSC were plated on human or murine stromal cells. In contrast, CD34⁺/CD10^-/CD19^-/CD45RA^- HSC plated on human or murine stromal cells expressed CD19 in both cultures, but TdT was only expressed in human stromal cell cultures. We conclude that human BM stromal cell, human skin fibroblasts, and murine S17 stromal cell cultures can provide complementary and comparative tools for identification of stromal cell ligands with potentially unique functions in regulating human B-cell development.

Original language	English (US)
Pages (from-to)	1271-1281
Number of pages	11
Journal	Experimental Hematology
Volume	27
Issue number	8
DOIs	https://doi.org/10.1016/S0301-472X(99)00067-3
State	Published - Aug 1999
Externally published	Yes

Bibliographical note

Funding Information:
We thank Janet Peller and the University of Minnesota Cancer Center Flow Cytometry Core Facility for cell sorting and for use of the FACSCalibur cytometers. We also thank Ted Bertrand for comments on the manuscript. This research was supported in part by Grants R01 CA31685, R01 CA76055, and P30 CA77598 from the National Cancer Institute/National Institutes of Health.

Keywords

B cell
Hematopoiesis
Stem cell
Stromal cell

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Comparative studies of different stromal cell microenvironments in support of human B-cell development",

abstract = "This study compared human murine stromal cells for their capacity to support human hematopoietic stem cell (HSC) development into the B lineage. FACS sorted human fetal bone marrow (BM) HSC (CD34+ CD19- or CD34+/CD10- /CD19-/CD45RA-) were cultured on human fetal BM stromal cells, human skin fibroblasts, or murine S17 stromal cells and analyzed by flow cytometry or reverse transcriptase polymerase chain reaction. CD34+CD19- HSC on human BM stromal cells or fibroblasts differentiated into B-lineage cells with a continuum in density of surface CD19 expression, and some cells expressing μ/κ or μ/λ B-cell receptors. In contrast, CD19+ cells from S17 cultures had two- to fourfold higher levels of CD19, but no cells expressing B-cell receptors. The number and percentage of CD19+ cells was high, intermediate, or low in the human BM, human fibroblast, or murine S17 stromal cell cultures, respectively. Reverse transcriptase polymerase chain reaction analysis showed that TdT, CD19, and D(H)Q52- J(H) rearrangements were expressed at comparable levels when CD34+/CD19- HSC were plated on human or murine stromal cells. In contrast, CD34+/CD10-/CD19-/CD45RA- HSC plated on human or murine stromal cells expressed CD19 in both cultures, but TdT was only expressed in human stromal cell cultures. We conclude that human BM stromal cell, human skin fibroblasts, and murine S17 stromal cell cultures can provide complementary and comparative tools for identification of stromal cell ligands with potentially unique functions in regulating human B-cell development.",

keywords = "B cell, Hematopoiesis, Stem cell, Stromal cell",

author = "Daitaro Kurosaka and Lebien, {Tucker W.} and Pribyl, {Julie A.R.}",

note = "Funding Information: We thank Janet Peller and the University of Minnesota Cancer Center Flow Cytometry Core Facility for cell sorting and for use of the FACSCalibur cytometers. We also thank Ted Bertrand for comments on the manuscript. This research was supported in part by Grants R01 CA31685, R01 CA76055, and P30 CA77598 from the National Cancer Institute/National Institutes of Health.",

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doi = "10.1016/S0301-472X(99)00067-3",

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AU - Kurosaka, Daitaro

AU - Lebien, Tucker W.

AU - Pribyl, Julie A.R.

N1 - Funding Information: We thank Janet Peller and the University of Minnesota Cancer Center Flow Cytometry Core Facility for cell sorting and for use of the FACSCalibur cytometers. We also thank Ted Bertrand for comments on the manuscript. This research was supported in part by Grants R01 CA31685, R01 CA76055, and P30 CA77598 from the National Cancer Institute/National Institutes of Health.

PY - 1999/8

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N2 - This study compared human murine stromal cells for their capacity to support human hematopoietic stem cell (HSC) development into the B lineage. FACS sorted human fetal bone marrow (BM) HSC (CD34+ CD19- or CD34+/CD10- /CD19-/CD45RA-) were cultured on human fetal BM stromal cells, human skin fibroblasts, or murine S17 stromal cells and analyzed by flow cytometry or reverse transcriptase polymerase chain reaction. CD34+CD19- HSC on human BM stromal cells or fibroblasts differentiated into B-lineage cells with a continuum in density of surface CD19 expression, and some cells expressing μ/κ or μ/λ B-cell receptors. In contrast, CD19+ cells from S17 cultures had two- to fourfold higher levels of CD19, but no cells expressing B-cell receptors. The number and percentage of CD19+ cells was high, intermediate, or low in the human BM, human fibroblast, or murine S17 stromal cell cultures, respectively. Reverse transcriptase polymerase chain reaction analysis showed that TdT, CD19, and D(H)Q52- J(H) rearrangements were expressed at comparable levels when CD34+/CD19- HSC were plated on human or murine stromal cells. In contrast, CD34+/CD10-/CD19-/CD45RA- HSC plated on human or murine stromal cells expressed CD19 in both cultures, but TdT was only expressed in human stromal cell cultures. We conclude that human BM stromal cell, human skin fibroblasts, and murine S17 stromal cell cultures can provide complementary and comparative tools for identification of stromal cell ligands with potentially unique functions in regulating human B-cell development.

AB - This study compared human murine stromal cells for their capacity to support human hematopoietic stem cell (HSC) development into the B lineage. FACS sorted human fetal bone marrow (BM) HSC (CD34+ CD19- or CD34+/CD10- /CD19-/CD45RA-) were cultured on human fetal BM stromal cells, human skin fibroblasts, or murine S17 stromal cells and analyzed by flow cytometry or reverse transcriptase polymerase chain reaction. CD34+CD19- HSC on human BM stromal cells or fibroblasts differentiated into B-lineage cells with a continuum in density of surface CD19 expression, and some cells expressing μ/κ or μ/λ B-cell receptors. In contrast, CD19+ cells from S17 cultures had two- to fourfold higher levels of CD19, but no cells expressing B-cell receptors. The number and percentage of CD19+ cells was high, intermediate, or low in the human BM, human fibroblast, or murine S17 stromal cell cultures, respectively. Reverse transcriptase polymerase chain reaction analysis showed that TdT, CD19, and D(H)Q52- J(H) rearrangements were expressed at comparable levels when CD34+/CD19- HSC were plated on human or murine stromal cells. In contrast, CD34+/CD10-/CD19-/CD45RA- HSC plated on human or murine stromal cells expressed CD19 in both cultures, but TdT was only expressed in human stromal cell cultures. We conclude that human BM stromal cell, human skin fibroblasts, and murine S17 stromal cell cultures can provide complementary and comparative tools for identification of stromal cell ligands with potentially unique functions in regulating human B-cell development.

KW - B cell

KW - Hematopoiesis

KW - Stem cell

KW - Stromal cell

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SN - 0301-472X

VL - 27

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JO - Experimental Hematology

JF - Experimental Hematology

IS - 8

ER -

Comparative studies of different stromal cell microenvironments in support of human B-cell development

Abstract

Bibliographical note

Keywords

UN SDGs

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