Conservation in Function of a SCAR/WAVE Component during Infection Thread and Root Hair Growth in Medicago truncatula

Akira Miyahara, Jennifer Richens, Colby Starker, Giulia Morieri, Lucinda Smith, Sharon Long, J. Allan Downie, Giles E.D. Oldroyd

Research output: Contribution to journalArticlepeer-review

51 Scopus citations

Abstract

Nitrogen-fixing symbioses of plants are often associated with bacterially infected nodules where nitrogen fixation occurs. The plant host facilitates bacterial infection with the formation of infection threads, unique structures associated with these symbioses, which are invaginations of the host cell with the capability of traversing cellular junctions. Here, we show that the infection thread shares mechanistic similarities to polar-growing cells, because the required for infection thread (RIT) locus of Medicago truncatula has roles in roothair, trichome, and infection-thread growth. We show that RIT encodes the M. truncatula ortholog of NAP1, a component of the SCAR/WAVE (suppressor of cAMP receptor/ WASP-family verprolin homologous protein) complex that regulates actin polymerization, through the activation of ARP2/3. NAP1 of Arabidopsis thaliana functions equivalently to the M. truncatula gene, indicating that the mode of action of NAP1 is functionally conserved across species and that legumes have not evolved a unique functionality for NAP1 during rhizobial colonization. This work highlights the surprising commonality between polar-growing cells and a polar-growing cellular intrusion and reveals important insights into the formation and maintenance of infectionthread development.

Original languageEnglish (US)
Pages (from-to)1553-1562
Number of pages10
JournalMolecular Plant-Microbe Interactions
Volume23
Issue number12
DOIs
StatePublished - Dec 2010

Fingerprint Dive into the research topics of 'Conservation in Function of a SCAR/WAVE Component during Infection Thread and Root Hair Growth in Medicago truncatula'. Together they form a unique fingerprint.

Cite this