Retinal S-antigen is widely used to study the LEW rat model of experimental autoimmune uveoretinitis (EAU). In this report, we have examined the T cell receptor V gene usage of several T cell lines recognizing either pathogenic or nonpathogenic sites on S-antigen to determine whether the Vα510 and Vβ510 rat homologues of the murine Vα2 and Vβ8 families, respectively, are used by uveitogenic T cells. Using cDNA probes for a LEW rat T cell receptor specific for the encephalitogenic determinant of myelin basic protein, we have found that in the retinal S-antigen/EAU model for autoimmune disease, pathogenicity correlates with usage of those rat V genes. Thus, all of the pathogenic lines were found to express T cell receptors of the Vβ510 and Vα510 families; conversely, Vβ510 usage was not detected in any of the nonpathogenic lines. Usage of these V regions has been associated with pathogenicity in the murine and rat models of experimental autoimmune encephalomyelitis, and now with S-antigen-induced EAU.
Bibliographical noteFunding Information:
’ This work was supported by NIH Grants EY05417 (D.S.G.) and NSllO86 (E.H.-K.) and the National Multiple Sclerosis Society (E.H.-K.). D.S.G. is a Research to Prevent Blindness Senior Scientific Investigator. * To whom correspondence should be addressed. 3 Abbreviations used: S-Ag, retinal S-antigen; EAU, experimental autoimmune uveoretinitis; EAE, experimental autoimmune encephalomyelitis; MBP, myelin basic protein; BSAg, bovine retinal S-antigen: HSAg, human retinal S-antigen; TCR, T cell antigen receptor.