Coordinated Muc2 and Muc3 mucin gene expression in Trichinella spiralis infection in wild-type and cytokine-deficient mice

L. L. Shekels, R. E. Anway, J. Lin, M. W. Kennedy, P. Garside, C. E. Lawrence, S. B. Ho

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

Mucin hypersecretion is an important component of the immune response to gastrointestinal nematode infection. Two discrete types of mucin proteins exist in the mouse intestine, secretory Muc2 and membrane-bound Muc3. We examined Muc2 and Muc3 expression in wild-type mice and mice lacking gamma interferon receptor (IFNγ,R-/-), tumor necrosis factor receptor 1 (TNFR1-/-) and interleukin 4 (IL4-/-) infected with Trichinella spiralis. Infected wild-type mice demonstrated significant goblet cell hyperplasia and increased mucin glycoprotein. In situ hybridization showed this was accompanied by increases in Muc2 and Muc3 mRNA. Total intestinal mucin protein and Muc2 and Muc3 mRNA levels were also significantly increased in cytokine-deficient mice. These data demonstrate the coordinated up-regulation of two types of mucin genes in response to T. spiralis infection and may form the basis of an innate mucosal response independent of IFN-γTNF, and IL-4.

Original languageEnglish (US)
Pages (from-to)1757-1764
Number of pages8
JournalDigestive Diseases and Sciences
Volume46
Issue number8
DOIs
StatePublished - 2001

Bibliographical note

Funding Information:
This work was supported by a Veterans Affairs Merit Review Award to S.B.H. and a Crohn’s and Colitis Career Development Award to L.L.S. This work was also supported by the Wellcome Trust—grant number 042679 to M.W.K. and grant number 055503 to C.E.L. P.G. is supported by a Wellcome Trust Career Development Fellowship.

Keywords

  • Cytokines
  • Immune response
  • Intestine
  • Mucin
  • Parasite
  • Trichinella spiralis

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