Coordinated Muc2 and Muc3 mucin gene expression in Trichinella spiralis infection in wild-type and cytokine-deficient mice

L. L. Shekels; R. E. Anway; J. Lin; M. W. Kennedy; P. Garside; C. E. Lawrence; S. B. Ho

doi:10.1023/A:1010622125040

Coordinated Muc2 and Muc3 mucin gene expression in Trichinella spiralis infection in wild-type and cytokine-deficient mice

L. L. Shekels, R. E. Anway, J. Lin, M. W. Kennedy, P. Garside, C. E. Lawrence, S. B. Ho

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

Mucin hypersecretion is an important component of the immune response to gastrointestinal nematode infection. Two discrete types of mucin proteins exist in the mouse intestine, secretory Muc2 and membrane-bound Muc3. We examined Muc2 and Muc3 expression in wild-type mice and mice lacking gamma interferon receptor (IFNγ,R-/-), tumor necrosis factor receptor 1 (TNFR1-/-) and interleukin 4 (IL4-/-) infected with Trichinella spiralis. Infected wild-type mice demonstrated significant goblet cell hyperplasia and increased mucin glycoprotein. In situ hybridization showed this was accompanied by increases in Muc2 and Muc3 mRNA. Total intestinal mucin protein and Muc2 and Muc3 mRNA levels were also significantly increased in cytokine-deficient mice. These data demonstrate the coordinated up-regulation of two types of mucin genes in response to T. spiralis infection and may form the basis of an innate mucosal response independent of IFN-γTNF, and IL-4.

Original language	English (US)
Pages (from-to)	1757-1764
Number of pages	8
Journal	Digestive Diseases and Sciences
Volume	46
Issue number	8
DOIs	https://doi.org/10.1023/A:1010622125040
State	Published - 2001

Bibliographical note

Funding Information:
This work was supported by a Veterans Affairs Merit Review Award to S.B.H. and a Crohn’s and Colitis Career Development Award to L.L.S. This work was also supported by the Wellcome Trust—grant number 042679 to M.W.K. and grant number 055503 to C.E.L. P.G. is supported by a Wellcome Trust Career Development Fellowship.

Keywords

Cytokines
Immune response
Intestine
Mucin
Parasite
Trichinella spiralis

Access

10.1023/A:1010622125040

OpenUrl availability

Full text

Cite this

@article{2f5e2eaa57974737b037cca1048e1c24,

title = "Coordinated Muc2 and Muc3 mucin gene expression in Trichinella spiralis infection in wild-type and cytokine-deficient mice",

abstract = "Mucin hypersecretion is an important component of the immune response to gastrointestinal nematode infection. Two discrete types of mucin proteins exist in the mouse intestine, secretory Muc2 and membrane-bound Muc3. We examined Muc2 and Muc3 expression in wild-type mice and mice lacking gamma interferon receptor (IFNγ,R-/-), tumor necrosis factor receptor 1 (TNFR1-/-) and interleukin 4 (IL4-/-) infected with Trichinella spiralis. Infected wild-type mice demonstrated significant goblet cell hyperplasia and increased mucin glycoprotein. In situ hybridization showed this was accompanied by increases in Muc2 and Muc3 mRNA. Total intestinal mucin protein and Muc2 and Muc3 mRNA levels were also significantly increased in cytokine-deficient mice. These data demonstrate the coordinated up-regulation of two types of mucin genes in response to T. spiralis infection and may form the basis of an innate mucosal response independent of IFN-γTNF, and IL-4.",

keywords = "Cytokines, Immune response, Intestine, Mucin, Parasite, Trichinella spiralis",

author = "Shekels, {L. L.} and Anway, {R. E.} and J. Lin and Kennedy, {M. W.} and P. Garside and Lawrence, {C. E.} and Ho, {S. B.}",

note = "Funding Information: This work was supported by a Veterans Affairs Merit Review Award to S.B.H. and a Crohn{\textquoteright}s and Colitis Career Development Award to L.L.S. This work was also supported by the Wellcome Trust—grant number 042679 to M.W.K. and grant number 055503 to C.E.L. P.G. is supported by a Wellcome Trust Career Development Fellowship.",

year = "2001",

doi = "10.1023/A:1010622125040",

language = "English (US)",

volume = "46",

pages = "1757--1764",

journal = "Digestive Diseases and Sciences",

issn = "0163-2116",

publisher = "Springer New York",

number = "8",

}

TY - JOUR

T1 - Coordinated Muc2 and Muc3 mucin gene expression in Trichinella spiralis infection in wild-type and cytokine-deficient mice

AU - Shekels, L. L.

AU - Anway, R. E.

AU - Lin, J.

AU - Kennedy, M. W.

AU - Garside, P.

AU - Lawrence, C. E.

AU - Ho, S. B.

N1 - Funding Information: This work was supported by a Veterans Affairs Merit Review Award to S.B.H. and a Crohn’s and Colitis Career Development Award to L.L.S. This work was also supported by the Wellcome Trust—grant number 042679 to M.W.K. and grant number 055503 to C.E.L. P.G. is supported by a Wellcome Trust Career Development Fellowship.

PY - 2001

Y1 - 2001

N2 - Mucin hypersecretion is an important component of the immune response to gastrointestinal nematode infection. Two discrete types of mucin proteins exist in the mouse intestine, secretory Muc2 and membrane-bound Muc3. We examined Muc2 and Muc3 expression in wild-type mice and mice lacking gamma interferon receptor (IFNγ,R-/-), tumor necrosis factor receptor 1 (TNFR1-/-) and interleukin 4 (IL4-/-) infected with Trichinella spiralis. Infected wild-type mice demonstrated significant goblet cell hyperplasia and increased mucin glycoprotein. In situ hybridization showed this was accompanied by increases in Muc2 and Muc3 mRNA. Total intestinal mucin protein and Muc2 and Muc3 mRNA levels were also significantly increased in cytokine-deficient mice. These data demonstrate the coordinated up-regulation of two types of mucin genes in response to T. spiralis infection and may form the basis of an innate mucosal response independent of IFN-γTNF, and IL-4.

AB - Mucin hypersecretion is an important component of the immune response to gastrointestinal nematode infection. Two discrete types of mucin proteins exist in the mouse intestine, secretory Muc2 and membrane-bound Muc3. We examined Muc2 and Muc3 expression in wild-type mice and mice lacking gamma interferon receptor (IFNγ,R-/-), tumor necrosis factor receptor 1 (TNFR1-/-) and interleukin 4 (IL4-/-) infected with Trichinella spiralis. Infected wild-type mice demonstrated significant goblet cell hyperplasia and increased mucin glycoprotein. In situ hybridization showed this was accompanied by increases in Muc2 and Muc3 mRNA. Total intestinal mucin protein and Muc2 and Muc3 mRNA levels were also significantly increased in cytokine-deficient mice. These data demonstrate the coordinated up-regulation of two types of mucin genes in response to T. spiralis infection and may form the basis of an innate mucosal response independent of IFN-γTNF, and IL-4.

KW - Cytokines

KW - Immune response

KW - Intestine

KW - Mucin

KW - Parasite

KW - Trichinella spiralis

UR - http://www.scopus.com/inward/record.url?scp=0034906538&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034906538&partnerID=8YFLogxK

U2 - 10.1023/A:1010622125040

DO - 10.1023/A:1010622125040

M3 - Article

C2 - 11508679

AN - SCOPUS:0034906538

SN - 0163-2116

VL - 46

SP - 1757

EP - 1764

JO - Digestive Diseases and Sciences

JF - Digestive Diseases and Sciences

IS - 8

ER -

Coordinated Muc2 and Muc3 mucin gene expression in Trichinella spiralis infection in wild-type and cytokine-deficient mice

Abstract

Bibliographical note

Keywords

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this