TY - JOUR
T1 - Crystal structure of the type III effector AvrB from Pseudomonas syringae
AU - Lee, Christian C.
AU - Wood, Michelle D.
AU - Ng, Kenneth
AU - Andersen, Carsten B.
AU - Liu, Yi
AU - Luginbühl, Peter
AU - Spraggon, Glen
AU - Katagiri, Fumiaki
PY - 2004/3
Y1 - 2004/3
N2 - AvrB is a Pseudomonas syringae type III effector protein that is translocated into host plant cells during attempted pathogenesis. Arabidopsis harboring the corresponding resistance protein RPM1 can detect AvrB and mount a rapid host defense response, thus avoiding active infection. In the plant cell, AvrB induces phosphorylation of RIN4, a key component in AvrB/RPM1 recognition. Although the AvrB/RPM1 system is among the best characterized of the numerous bacterial effector/plant resistance protein systems involved in plant disease resistance and pathogenesis, the details of the molecular recognition mechanism are still unclear. To gain further insights, the crystal structure of AvrB was determined. The 2.2 Å structure exhibits a novel mixed α/β bilobal fold. Aided by the structural information, we demonstrate that one lobe is the determinant of AvrB/RPM1 recognition specificity. This structural information and preliminary structure-function studies provide a framework for the future understanding of AvrB function on the molecular level.
AB - AvrB is a Pseudomonas syringae type III effector protein that is translocated into host plant cells during attempted pathogenesis. Arabidopsis harboring the corresponding resistance protein RPM1 can detect AvrB and mount a rapid host defense response, thus avoiding active infection. In the plant cell, AvrB induces phosphorylation of RIN4, a key component in AvrB/RPM1 recognition. Although the AvrB/RPM1 system is among the best characterized of the numerous bacterial effector/plant resistance protein systems involved in plant disease resistance and pathogenesis, the details of the molecular recognition mechanism are still unclear. To gain further insights, the crystal structure of AvrB was determined. The 2.2 Å structure exhibits a novel mixed α/β bilobal fold. Aided by the structural information, we demonstrate that one lobe is the determinant of AvrB/RPM1 recognition specificity. This structural information and preliminary structure-function studies provide a framework for the future understanding of AvrB function on the molecular level.
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U2 - 10.1016/j.str.2004.02.013
DO - 10.1016/j.str.2004.02.013
M3 - Article
C2 - 15016364
AN - SCOPUS:1542476961
SN - 0969-2126
VL - 12
SP - 487
EP - 494
JO - Structure
JF - Structure
IS - 3
ER -