Curvature Elasticity-Driven Leaflet Asymmetry and Interleaflet Raft Coupling in Supported Membranes

Yong Sang Ryu, Luke R. Jordan, Nathan J. Wittenberg, Sang Moon Kim, Daehan Yoo, Cherlhyun Jeong, Arthur E. Warrington, Moses Rodriguez, Sang Hyun Oh, Sin Doo Lee

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Membrane curvature is closely associated with spatial distribution of lipids and curvature can influence the formation of lipid rafts in biological membranes. However, it is still unclear how the curvature elasticity plays a role on the asymmetric distribution of lipids across the membrane leaflets, especially, during the process of the raft domain growth. Using supported lipid membranes on wedge-shaped substrates with alternating positive and negative curvatures, curvature-mediated asymmetry of lipid raft domains is observed across the membrane leaflets accompanied by glycolipid receptor (GM1 and GT1b) localization. The raft domains initially appear only in a membrane leaflet possessing negative curvatures. In the presence of the interleaflet coupling, they evolve to generate the transverse registry across the membrane bilayer. It is shown that a human recombinant antibody rHIgM12 is co-localized with the rafts formed at the peaks and valleys of the wedge substrate, indicating that the spatial distribution of its receptor (GT1b) is indeed manifested by the site-specific formation of asymmetric raft domains through the curvature elasticity. The platform can be used to explore the mechanism for the leaflet asymmetry and lipid sorting in terms of the membrane curvature, the composition, and the receptor presentation.

Original languageEnglish (US)
Article number1801290
JournalAdvanced Materials Interfaces
Volume5
Issue number23
DOIs
StatePublished - Dec 7 2018

Bibliographical note

Funding Information:
The authors would like to thank the technical staffs of Inter-University Semiconductor Research Center at Seoul National University for technical assistance. This work was supported in part by the U.S. National Science Foundation (NSF CAREER award), the National Institutes of Health (R01 GM092993), the MnDRIVE Initiative, and the Minnesota Partnership for Biotechnology and Medical Genomics. S.D.L. acknowledges the support by the BK21 Plus Program of Ministry of Education of Korea and Y.S.R. acknowledges the support by KIST intramural grants with No. 2E28390 and 2E28262. N.J.W. acknowledges support from Lehigh University.

Publisher Copyright:
© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

Keywords

  • curvature
  • interleaflet raft coupling
  • lipid membranes
  • lipid raft
  • phase separation

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