Cytotoxicity of fumonisin B1: Implication of lipid peroxidation and inhibition of protein and DNA syntheses

Karine Abado-Becognee, Théophile Amondo Mobio, Rachid Ennamany, Francis Fleurat-Lessard, W. T. Shier, F. Badria, Edmond Ekué Creppy

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86 Scopus citations

Abstract

The effects of fumonisin B1 (FB1) from Fusarium moniliforme on lipid peroxidation and protein and DNA syntheses were studied in monkey kidney cells (Vero cells). FB1 was found to be a potent inducer of malondialdehyde (MDA), one of the secondary products formed during lipid peroxidation. At 0.14 μM (0.1 μg/ml), FB1 induced 0.496 ± 0.1 nmoles of MDA/ mg protein, compared to the control level 0.134 ± 0.01 nmoles of MDA/mg protein (P < 0.005). No inhibition of protein or DNA synthesis was observed at this concentration of FB1. Inhibition of protein and DNA syntheses was observed at FB1 concentrations > 14 μM C10 μg/ml) with an IC50 of 33 μM for both protein synthesis and DNA synthesis. These results indicate that lipid peroxidation is a very sensitive cellular response to the mycotoxin fumonisin B1 observed at concentrations lower than that required to inhibit cellular synthesis of macromolecules, protein and DNA. This oxidative damage induced by FB1 concentrations encountered in naturally contaminated foodstuffs and feed might lead to mutagenicity and genotoxicity.

Original languageEnglish (US)
Pages (from-to)233-236
Number of pages4
JournalArchives of Toxicology
Volume72
Issue number4
DOIs
StatePublished - Jan 1 1998

Keywords

  • Fusarium moniliformeFumonisin B
  • Lipid peroxidation
  • Malondialdehyde
  • Protein and DNA syntheses

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