Deleterious effects of endotoxin on cultured endothelial cells: An in vitro model of vascular injury

Osamu Yamada, Charles F. Moldow, Thomas Sacks, Philip R. Craddock, M. A. Boogaerts, Harry S Jacob

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

The effects of endotoxin-triggered granulocytes on the viability of endothelial cells in vitro was investigated. Endotoxin or its lipid A component caused granulocytes to adhere to and significantly damage cultured endothelial cells. Fresh serum is not necessary but does amplify both adherence and endothelial injury. Much of the endothelial injury was inhibited by free-radical scavengers or by blocking granulocyte adhesion to endothelial cells and appears to result from free radical production by the stimulated granulocyte. Studies in this model suggest a pathogenic role for the endotoxin-triggered granulocyte in the Shwartzman reaction and perhaps related clinical disorders.

Original languageEnglish (US)
Pages (from-to)115-126
Number of pages12
JournalInflammation
Volume5
Issue number2
DOIs
StatePublished - Jun 1 1981

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