Delta-like 1 protein, Vitamin D binding protein and fetuin for detection of Mycobacterium tuberculosis meningitis

Nathan C Bahr, Ryan Halupnick, Grace Linder, Reuben Kiggundu, Henry W. Nabeta, Darlisha A. Williams, Abdu K. Musubire, Bozena M. Morawski, Srinand Sreevatsan, David B. Meya, Joshua R Rhein, David R Boulware

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Aim: Tuberculosis meningitis (TBM) diagnosis is difficult, new biomarkers are needed. We evaluated the diagnostic utility of delta-like 1 protein (DLL1), vitamin D binding protein (VDBP) and fetuin. Methods: Biomarker concentrations were measured by ELISA in cryopreserved cerebrospinal fluid from 139 HIV-infected Ugandans with suspected meningitis. TBM was diagnosed by GeneXpert MTB/Rif or culture. Cohort diagnoses included TBM (n = 22), cryptococcal (n = 71), or aseptic meningitis (n = 16) and no meningitis (n = 30). Results: DLL1 (cut-off value 1150 pg/ml) provided 32% sensitivity and 98% specificity. Adding fetuin, cryptococcal antigen and IFN-γ resulted in sensitivities of 36, 63 and 76% with specificities of 98, 90 and 92%, respectively. VDBP (cut-off value 2.0 μg/ml) provided 81% sensitivity and 68% specificity while fetuin (cut-off value 2 μg/ml) provided a sensitivity of 86% and specificity of 68%. Conclusion: CSF DLL1, VDBP and fetuin exhibited fair diagnostic performance for TBM diagnosis.

Original languageEnglish (US)
Pages (from-to)707-716
Number of pages10
JournalBiomarkers in Medicine
Volume12
Issue number7
DOIs
StatePublished - Jul 2018

Bibliographical note

Funding Information:
This research was made possible through support from the Fogarty International Center (R25TW009345), the National Institute of Neurologic Diseases and Stroke (R01NS086312) and the National Institute of Allergy and Infectious Diseases (U01AI089244). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript.

Publisher Copyright:
© 2018 2018 Future Medicine Ltd.

Keywords

  • Mycobacterium tuberculosis
  • TB meningitis
  • biomarkers

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