Delta-opioid receptor mrna expression and immunohistochemical localization in porcine ileum

David R. Brown; Sutthasinee Poonyachoti; Mark A. Osinski; Teresa R. Kowalski; Mary S. Pampusch; Robert P. Elde; Michael P. Murtaugh

doi:10.1023/A:1018877823534

Delta-opioid receptor mrna expression and immunohistochemical localization in porcine ileum

David R. Brown, Sutthasinee Poonyachoti, Mark A. Osinski, Teresa R. Kowalski, Mary S. Pampusch, Robert P. Elde, Michael P. Murtaugh

Veterinary and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Opiates have potent antidiarrheal actions that are mediated in part by delta-opioid receptors (DOR). We examined DOR localization within subregions of porcine ileum, a tissue analogous to human small bowel. A partial cDNA sequence for porcine DOR was obtained after reverse transcription-polymerase chain reaction cloning of forebrain RNA; it encoded the end of transmembrane domain 1 through the beginning of transmembrane domain 7 and exhibited 93% nucleotide identity with human DOR. Positive signals for DOR mRNA were found in all subregions of the porcine ileal wall. With an antiserum recognizing an N-terminal epitope in murine DOR, DOR-like immunoreactivity was localized in neurons within myenteric and submucous ganglia, longitudinal and circular smooth muscle, and villous lamina propria. The DOR agonist [D-Ser², Leu⁵, Thr⁶]enkephalin (DSLET) attenuated circular smooth muscle contractions in porcine ileum that were evoked by electrical stimulation of myenteric cholinergic neurons. These results are consistent with previous reports of the DOR-mediated neuromodulation that underlies the antipropulsive and antisecretory effects of opioids in the intestinal tract.

Original language	English (US)
Pages (from-to)	1402-1410
Number of pages	9
Journal	Digestive Diseases and Sciences
Volume	43
Issue number	7
DOIs	https://doi.org/10.1023/A:1018877823534
State	Published - 1998

Bibliographical note

Funding Information:
Supported in part by a University of Minnesota Graduate School Grant-in-Aid of Research # 16411 to D.R.B., and National Institutes on Drug Abuse grants DA-10200 to D.R.B.; DA-09643 to R.P.E., and DA-08010 to M.P.M. Support for S.P., M.A.O., and M.S.P. was provided by a RoyaThlaiGove rnme nt scholarship, Ne uroscience Training in Drug Abuse Research grant DA-07234 and Training in Psychone uroimmunology and Substance Abuse grant DA-07239, respectively.

Keywords

Host defense
Motility
Opioid receptor
Pig
Small intestine

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title = "Delta-opioid receptor mrna expression and immunohistochemical localization in porcine ileum",

abstract = "Opiates have potent antidiarrheal actions that are mediated in part by delta-opioid receptors (DOR). We examined DOR localization within subregions of porcine ileum, a tissue analogous to human small bowel. A partial cDNA sequence for porcine DOR was obtained after reverse transcription-polymerase chain reaction cloning of forebrain RNA; it encoded the end of transmembrane domain 1 through the beginning of transmembrane domain 7 and exhibited 93% nucleotide identity with human DOR. Positive signals for DOR mRNA were found in all subregions of the porcine ileal wall. With an antiserum recognizing an N-terminal epitope in murine DOR, DOR-like immunoreactivity was localized in neurons within myenteric and submucous ganglia, longitudinal and circular smooth muscle, and villous lamina propria. The DOR agonist [D-Ser2, Leu5, Thr6]enkephalin (DSLET) attenuated circular smooth muscle contractions in porcine ileum that were evoked by electrical stimulation of myenteric cholinergic neurons. These results are consistent with previous reports of the DOR-mediated neuromodulation that underlies the antipropulsive and antisecretory effects of opioids in the intestinal tract.",

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author = "Brown, {David R.} and Sutthasinee Poonyachoti and Osinski, {Mark A.} and Kowalski, {Teresa R.} and Pampusch, {Mary S.} and Elde, {Robert P.} and Murtaugh, {Michael P.}",

note = "Funding Information: Supported in part by a University of Minnesota Graduate School Grant-in-Aid of Research # 16411 to D.R.B., and National Institutes on Drug Abuse grants DA-10200 to D.R.B.; DA-09643 to R.P.E., and DA-08010 to M.P.M. Support for S.P., M.A.O., and M.S.P. was provided by a RoyaThlaiGove rnme nt scholarship, Ne uroscience Training in Drug Abuse Research grant DA-07234 and Training in Psychone uroimmunology and Substance Abuse grant DA-07239, respectively.",

year = "1998",

doi = "10.1023/A:1018877823534",

language = "English (US)",

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AU - Poonyachoti, Sutthasinee

AU - Osinski, Mark A.

AU - Kowalski, Teresa R.

AU - Pampusch, Mary S.

AU - Elde, Robert P.

AU - Murtaugh, Michael P.

N1 - Funding Information: Supported in part by a University of Minnesota Graduate School Grant-in-Aid of Research # 16411 to D.R.B., and National Institutes on Drug Abuse grants DA-10200 to D.R.B.; DA-09643 to R.P.E., and DA-08010 to M.P.M. Support for S.P., M.A.O., and M.S.P. was provided by a RoyaThlaiGove rnme nt scholarship, Ne uroscience Training in Drug Abuse Research grant DA-07234 and Training in Psychone uroimmunology and Substance Abuse grant DA-07239, respectively.

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N2 - Opiates have potent antidiarrheal actions that are mediated in part by delta-opioid receptors (DOR). We examined DOR localization within subregions of porcine ileum, a tissue analogous to human small bowel. A partial cDNA sequence for porcine DOR was obtained after reverse transcription-polymerase chain reaction cloning of forebrain RNA; it encoded the end of transmembrane domain 1 through the beginning of transmembrane domain 7 and exhibited 93% nucleotide identity with human DOR. Positive signals for DOR mRNA were found in all subregions of the porcine ileal wall. With an antiserum recognizing an N-terminal epitope in murine DOR, DOR-like immunoreactivity was localized in neurons within myenteric and submucous ganglia, longitudinal and circular smooth muscle, and villous lamina propria. The DOR agonist [D-Ser2, Leu5, Thr6]enkephalin (DSLET) attenuated circular smooth muscle contractions in porcine ileum that were evoked by electrical stimulation of myenteric cholinergic neurons. These results are consistent with previous reports of the DOR-mediated neuromodulation that underlies the antipropulsive and antisecretory effects of opioids in the intestinal tract.

AB - Opiates have potent antidiarrheal actions that are mediated in part by delta-opioid receptors (DOR). We examined DOR localization within subregions of porcine ileum, a tissue analogous to human small bowel. A partial cDNA sequence for porcine DOR was obtained after reverse transcription-polymerase chain reaction cloning of forebrain RNA; it encoded the end of transmembrane domain 1 through the beginning of transmembrane domain 7 and exhibited 93% nucleotide identity with human DOR. Positive signals for DOR mRNA were found in all subregions of the porcine ileal wall. With an antiserum recognizing an N-terminal epitope in murine DOR, DOR-like immunoreactivity was localized in neurons within myenteric and submucous ganglia, longitudinal and circular smooth muscle, and villous lamina propria. The DOR agonist [D-Ser2, Leu5, Thr6]enkephalin (DSLET) attenuated circular smooth muscle contractions in porcine ileum that were evoked by electrical stimulation of myenteric cholinergic neurons. These results are consistent with previous reports of the DOR-mediated neuromodulation that underlies the antipropulsive and antisecretory effects of opioids in the intestinal tract.

KW - Host defense

KW - Motility

KW - Opioid receptor

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JO - Digestive Diseases and Sciences

JF - Digestive Diseases and Sciences

IS - 7

ER -

Delta-opioid receptor mrna expression and immunohistochemical localization in porcine ileum

Abstract

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Keywords

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