Design and performance of telescoping micropipette arrays for high throughput in vivo patch clamping

Suhasa B. Kodandaramaiah, Saifullah Malik, Mike J. Dergance, Edward S. Boyden, Craig R. Forest

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Precise positioning (e.g., 1-100 μm) of an electrode or arrays of electrodes is a critical requirement for many in vivo electrophysiology studies. We are developing an instrument to manipulate arrays of micropipette electrodes utilizing a novel actuation strategy wherein flexible fused silica micropipettes telescope within teflon conduits configured in non-rectilinear configurations. This strategy circumvents the steric hindrances that limit the number micropipettes to just one in conventional setups. To prove this concept, we have designed a device that allows the simultaneous actuation of an array of four micropipettes. We have experimentally characterized the linear translation of micropipettes in this device and have achieved a minimum step resolution of 1 μm. Further in vivo electrophysiology experiments validate the efficacy of this device for in vivo patch clamping.

Original languageEnglish (US)
Title of host publicationProceedings - ASPE 2010 Annual Meeting
Pages246-249
Number of pages4
Volume50
StatePublished - Dec 1 2010
Event25th Annual Meeting of the American Society for Precision Engineering, ASPE 2010 - Atlanta, GA, United States
Duration: Oct 31 2010Nov 4 2010

Other

Other25th Annual Meeting of the American Society for Precision Engineering, ASPE 2010
CountryUnited States
CityAtlanta, GA
Period10/31/1011/4/10

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