Design and performance of telescoping micropipette arrays for high throughput in vivo patch clamping

Suhasa B. Kodandaramaiah; Saifullah Malik; Mike J. Dergance; Edward S. Boyden; Craig R. Forest

Design and performance of telescoping micropipette arrays for high throughput in vivo patch clamping

Suhasa B. Kodandaramaiah, Saifullah Malik, Mike J. Dergance, Edward S. Boyden, Craig R. Forest

Mechanical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Precise positioning (e.g., 1-100 μm) of an electrode or arrays of electrodes is a critical requirement for many in vivo electrophysiology studies. We are developing an instrument to manipulate arrays of micropipette electrodes utilizing a novel actuation strategy wherein flexible fused silica micropipettes telescope within teflon conduits configured in non-rectilinear configurations. This strategy circumvents the steric hindrances that limit the number micropipettes to just one in conventional setups. To prove this concept, we have designed a device that allows the simultaneous actuation of an array of four micropipettes. We have experimentally characterized the linear translation of micropipettes in this device and have achieved a minimum step resolution of 1 μm. Further in vivo electrophysiology experiments validate the efficacy of this device for in vivo patch clamping.

Original language	English (US)
Title of host publication	Proceedings - ASPE 2010 Annual Meeting
Pages	246-249
Number of pages	4
Volume	50
State	Published - Dec 1 2010
Event	25th Annual Meeting of the American Society for Precision Engineering, ASPE 2010 - Atlanta, GA, United States Duration: Oct 31 2010 → Nov 4 2010

Other

Other	25th Annual Meeting of the American Society for Precision Engineering, ASPE 2010
Country/Territory	United States
City	Atlanta, GA
Period	10/31/10 → 11/4/10

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title = "Design and performance of telescoping micropipette arrays for high throughput in vivo patch clamping",

abstract = "Precise positioning (e.g., 1-100 μm) of an electrode or arrays of electrodes is a critical requirement for many in vivo electrophysiology studies. We are developing an instrument to manipulate arrays of micropipette electrodes utilizing a novel actuation strategy wherein flexible fused silica micropipettes telescope within teflon conduits configured in non-rectilinear configurations. This strategy circumvents the steric hindrances that limit the number micropipettes to just one in conventional setups. To prove this concept, we have designed a device that allows the simultaneous actuation of an array of four micropipettes. We have experimentally characterized the linear translation of micropipettes in this device and have achieved a minimum step resolution of 1 μm. Further in vivo electrophysiology experiments validate the efficacy of this device for in vivo patch clamping.",

author = "Kodandaramaiah, {Suhasa B.} and Saifullah Malik and Dergance, {Mike J.} and Boyden, {Edward S.} and Forest, {Craig R.}",

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T1 - Design and performance of telescoping micropipette arrays for high throughput in vivo patch clamping

AU - Kodandaramaiah, Suhasa B.

AU - Malik, Saifullah

AU - Dergance, Mike J.

AU - Boyden, Edward S.

AU - Forest, Craig R.

PY - 2010/12/1

Y1 - 2010/12/1

N2 - Precise positioning (e.g., 1-100 μm) of an electrode or arrays of electrodes is a critical requirement for many in vivo electrophysiology studies. We are developing an instrument to manipulate arrays of micropipette electrodes utilizing a novel actuation strategy wherein flexible fused silica micropipettes telescope within teflon conduits configured in non-rectilinear configurations. This strategy circumvents the steric hindrances that limit the number micropipettes to just one in conventional setups. To prove this concept, we have designed a device that allows the simultaneous actuation of an array of four micropipettes. We have experimentally characterized the linear translation of micropipettes in this device and have achieved a minimum step resolution of 1 μm. Further in vivo electrophysiology experiments validate the efficacy of this device for in vivo patch clamping.

AB - Precise positioning (e.g., 1-100 μm) of an electrode or arrays of electrodes is a critical requirement for many in vivo electrophysiology studies. We are developing an instrument to manipulate arrays of micropipette electrodes utilizing a novel actuation strategy wherein flexible fused silica micropipettes telescope within teflon conduits configured in non-rectilinear configurations. This strategy circumvents the steric hindrances that limit the number micropipettes to just one in conventional setups. To prove this concept, we have designed a device that allows the simultaneous actuation of an array of four micropipettes. We have experimentally characterized the linear translation of micropipettes in this device and have achieved a minimum step resolution of 1 μm. Further in vivo electrophysiology experiments validate the efficacy of this device for in vivo patch clamping.

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T2 - 25th Annual Meeting of the American Society for Precision Engineering, ASPE 2010

Y2 - 31 October 2010 through 4 November 2010

ER -

Design and performance of telescoping micropipette arrays for high throughput in vivo patch clamping

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