TY - JOUR
T1 - Detection and quantitation of radiolabeled proteins in gels and blots
AU - Voytas, D.
AU - Ke, N.
PY - 2001/5/1
Y1 - 2001/5/1
N2 - This unit provides procedures for detecting an quantifying radiolabeled proteins in SDS-polyacrylamide gels or blots. Autoradiography requires fixed, dried gels before exposure to X-ray film. The results may be enhanced by using intensifying screens and/or preflashing the film. Alternatively fluorography with organic scintillant-impregnated gels may be used to enhance signal. The autoradiogram may be quantified by densitometry. Phosphorimaging is a useful alternative to film exposure.
AB - This unit provides procedures for detecting an quantifying radiolabeled proteins in SDS-polyacrylamide gels or blots. Autoradiography requires fixed, dried gels before exposure to X-ray film. The results may be enhanced by using intensifying screens and/or preflashing the film. Alternatively fluorography with organic scintillant-impregnated gels may be used to enhance signal. The autoradiogram may be quantified by densitometry. Phosphorimaging is a useful alternative to film exposure.
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U2 - 10.1002/0471140856.txa03ds07
DO - 10.1002/0471140856.txa03ds07
M3 - Article
C2 - 20972964
AN - SCOPUS:84925548128
SN - 1934-9254
VL - Appendix 3
JO - Current protocols in toxicology / editorial board, Mahin D. Maines (editor-in-chief) ... [et al.]
JF - Current protocols in toxicology / editorial board, Mahin D. Maines (editor-in-chief) ... [et al.]
ER -