Abstract
Objectives: To develop an HPLC method for the analysis of trazodone and its metabolite, 1-m-chlorophenyl-piperazine (m-CPP), in human plasma and red blood cells. Design and Methods: The analytes were extracted by heptane containing 1.5% isoamyl alcohol, back extracted into phosphoric acid and analyzed by reverse phase HPLC with UV detection. Results: In seven randomly selected, male, human subjects plasma concentrations (nmol/l) were 380-5841 for trazodone and 14-237 for rn-CPP while those in packed red blood cells were 98-634 for trazodone and 15-155 for m-CPP. Plasma trazodone concentrations were 4-11-fold higher than those in red blood cells while those of m-CPP were about equal. This may be the first report on concentrations of trazodone and m-CPP in human red blood cells. Conclusions: This sensitive method can be used for monitoring trazodone and m-CPP in human plasma and red blood cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 149-153 |
| Number of pages | 5 |
| Journal | Clinical Biochemistry |
| Volume | 30 |
| Issue number | 2 |
| DOIs | |
| State | Published - Mar 1 1997 |
Keywords
- and erythrocytes
- antidepressive agents
- high pressure liquid chromatography
- piperazines
- plasma
- trazodone