Determining Direction of Axonal Flow in the Equine Ramus Communicans by Ultrastructural Examination of the Plantar Nerves 2 Months after Transecting the Ramus

Fakhri Al-Bagdadi, Jim Schumacher, Jessi Carter, Ferenc Tóth, Robert W. Henry

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The ramus communicans, neural connection between medial and lateral plantar nerves of the horse, was transected to determine the degree to which medial and lateral plantar nerves contribute to the plantar ramus. After 2 months, sections of plantar nerves immediately proximal and distal to the communicating branch were collected and processed for electron microscopy. All examined nerves had undergone Wallerian degeneration and contained regenerating and mature fibers. Layers of the myelin sheath were separated by spaces and vacuoles, indicating demyelination of medial and lateral plantar nerves. Shrunken axons varied in diameter and were surrounded by an irregular axolemma. Shrunken axoplasm of both myelinated and non-myelinated fibers contained ruptured mitochondria and cristae, disintegrating cytoskeleton, and vacuoles of various sizes. The cytoplasm of neurolemmocytes contained various-sized vesicles, ruptured mitochondria within a fragile basal lamina and myelin whorls of multilayered structures indicative of Wallerian degeneration. These ultrastructural changes, found proximal and distal to the ramus in medial and lateral plantar nerves, suggest that axonal flow is bi-directional through the ramus communicans of the pelvic limbs of horses, a previously unreported finding. As well, maturity of nerves proximal and distal to the ramus indicates that all nerve fibers do not pass through the ramus.

Original languageEnglish (US)
Pages (from-to)64-68
Number of pages5
JournalMicroscopy and Microanalysis
Volume24
Issue number1
DOIs
StatePublished - Feb 1 2018

Bibliographical note

Funding Information:
This research was supported by: Louisiana State University, School of Veterinary Medicine; University of Tennessee, College of Veterinary Medicine; and Lincoln Memorial University, College of Veterinary Medicine, TN. Dr. Hasan Zaman, LSU School of Veterinary Medicine, formatted manuscript for publication.

Publisher Copyright:
© Microscopy Society of America 2018.

Keywords

  • degeneration
  • equine
  • plantar nerve
  • ramus communicans
  • regeneration

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